Biphasic transitions of a hairpin hexanucleotide triplex DNA
文献类型：期刊论文
| 作者 | Lee, IS; Deng, WL; Yang, LJ; Wang, C; Bai, CL |
| 刊名 | BIOPHYSICAL CHEMISTRY
 |
| 出版日期 | 1997-09-01 |
| 卷号 | 67期号:1-3页码:159-165 |
| 关键词 | Conformational Transitions Hairpin Triplex Dna Uv Spectroscopy Electrostatic Effects |
| ISSN号 | 0301-4622 |
| 英文摘要 | The conformational transitions (helix-coil transitions) of three hairpin triple helices, models 5'-(A-G)(3) + 5'-(T-C)(3)-T-4- (C-br-T)(3) [CY], 5'-(A-G)(3) + 5'-(T-C-br)(3)-T-4-(C-T)(3) [YC] and 5'-(A-G)(3) + 5'-(T-C-br)(3)-T-4-(C-br-T)(3) [YY], are characterized in this work by UV spectroscopy. Melting of these triplexes is biphasic, and the profiles are used to obtain the thermodynamic parameters, The thermodynamic properties of the hairpin tripler are T-m = 19.45 degrees C and Delta H-vH = 293.12 kJ mol(-1) for CY, T-m = 22.85 degrees C and Delta H-vH = 256.63 kJ mol(-1) for YC and T-m = 28.47 degrees C and Delta H-vH = 234.68 kJ mol(-1) for YY at pH 4.4. Those of the duplex are T-m = 30.50 degrees C and Delta H-vH = 427.09 kJ mol(-1) for CY, T-m = 32.96 degrees C and Delta H-vH = 374.47 kJ mol(-1) for YC and T-m = 33.24 degrees C and Delta H-vH = 329.67 kJ mol(-1) for YY at pH 4.4. The distinct transitions of tripler to duplex and duplex to single strands are analyzed using the nearest-neighbor Ising model. Electrostatic effects on each conformation are also analyzed. (C) 1997 Elsevier Science B.V. |
| 语种 | 英语 |
| WOS记录号 | WOS:A1997YH20600016 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/44847]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Lee, IS |
| 推荐引用方式 GB/T 7714 | Lee, IS,Deng, WL,Yang, LJ,et al. Biphasic transitions of a hairpin hexanucleotide triplex DNA[J]. BIOPHYSICAL CHEMISTRY,1997,67(1-3):159-165. |
| APA | Lee, IS,Deng, WL,Yang, LJ,Wang, C,&Bai, CL.(1997).Biphasic transitions of a hairpin hexanucleotide triplex DNA.BIOPHYSICAL CHEMISTRY,67(1-3),159-165. |
| MLA | Lee, IS,et al."Biphasic transitions of a hairpin hexanucleotide triplex DNA".BIOPHYSICAL CHEMISTRY 67.1-3(1997):159-165. |
入库方式： OAI收割
来源：化学研究所
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