Laser-induced fluorescence detection of trace amino acids in rat brain microdialysates.
文献类型:期刊论文
作者 | Han, HW; Ma, MS; Xiong, SX; Liu, GQ |
刊名 | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
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出版日期 | 1997 |
卷号 | 24期号:5页码:461-464 |
关键词 | Microdialysates Laser-induced Fluorescence Detection Capillary Electrophoresis Amino Acid Learning |
ISSN号 | 1000-3282 |
英文摘要 | Trace amount of free amino acids In brain microdialysates was separated and determinated with derivative of fluorescein isothiocyanate (FITC) using the home-made microdialysis probes and biochemical sampling unit for living animal as well as capillary electrophoresis system with intensified charge-coupled device (ICCD)-laser Induced fluorescence detector. The concentration of free amino acids in microdialysates was 10(-8) similar to 10(-6) mol/L with 5 mu l sample. The change of free amino acids was monitored in vivo in the hippocampus of freely moving rats before and after operational learning. These experiments suggest that the sampling and determination system can be used for analysis of amino acids in brain microdialysates. |
语种 | 英语 |
WOS记录号 | WOS:A1997YF17600019 |
出版者 | SCIENCE CHINA PRESS |
源URL | [http://ir.iccas.ac.cn/handle/121111/44859] ![]() |
专题 | 中国科学院化学研究所 |
作者单位 | CHINESE ACAD SCI,INST CHEM,BEIJING 100080,PEOPLES R CHINA |
推荐引用方式 GB/T 7714 | Han, HW,Ma, MS,Xiong, SX,et al. Laser-induced fluorescence detection of trace amino acids in rat brain microdialysates.[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,1997,24(5):461-464. |
APA | Han, HW,Ma, MS,Xiong, SX,&Liu, GQ.(1997).Laser-induced fluorescence detection of trace amino acids in rat brain microdialysates..PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,24(5),461-464. |
MLA | Han, HW,et al."Laser-induced fluorescence detection of trace amino acids in rat brain microdialysates.".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 24.5(1997):461-464. |
入库方式: OAI收割
来源:化学研究所
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