中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
DSC study of cold and heat denaturation processes of beta-lactoglobulin A with guanidine hydrochloride

文献类型:期刊论文

作者Wang, BN; Tan, F
刊名SCIENCE IN CHINA SERIES B-CHEMISTRY
出版日期1997-06-01
卷号40期号:3页码:316-322
关键词Cold Denaturation Heat Denaturation Beta-lactoglobulin a Guanidine Hydrochloride Differential Scanning Calorimetry
ISSN号1001-652X
英文摘要The cold and heat denaturations of bovine beta-lactoglobulin A (beta-lg A) has been studied in solutions of guanidine hydrochloride (GuHCl) by differential scanning calorimetry (DSC). The experimental results are presented and discussed. It is shown that the number of protons bound by the monomeric molecules of beta-lg A was unchanged before and after its heat denaturation below pH 3, and that the activation energy of the heat denaturation was depressed owing to the presence of GuHCl. In the solutions with 2.50 and 3.06 mol/L of GuHCl, both the cold and heat denaturations of beta-lg A were observed. In comparison with the heat denaturation, the activation energy of cold denaturation was far lower and the number of GuHCl molecules bound by the unfolded polypeptide chains after cold denaturation increased a lot. The absolute value of the enthalpy of cold denaturation was larger than that of heat denaturation. It was found by the analysis that the contribution to the total denaturational enthalpy of conformational change itself of the monomeric molecules of beta-lg A was the lowest among the globulins, according to the average of the number of heavy atoms.
语种英语
WOS记录号WOS:A1997XB99000012
出版者SCIENCE CHINA PRESS
源URL[http://ir.iccas.ac.cn/handle/121111/44879]  
专题中国科学院化学研究所
通讯作者Wang, BN
推荐引用方式
GB/T 7714
Wang, BN,Tan, F. DSC study of cold and heat denaturation processes of beta-lactoglobulin A with guanidine hydrochloride[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,1997,40(3):316-322.
APA Wang, BN,&Tan, F.(1997).DSC study of cold and heat denaturation processes of beta-lactoglobulin A with guanidine hydrochloride.SCIENCE IN CHINA SERIES B-CHEMISTRY,40(3),316-322.
MLA Wang, BN,et al."DSC study of cold and heat denaturation processes of beta-lactoglobulin A with guanidine hydrochloride".SCIENCE IN CHINA SERIES B-CHEMISTRY 40.3(1997):316-322.

入库方式: OAI收割

来源:化学研究所

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