A Strategy for Specific Fluorescence Imaging of Monoamine OxidaseA in Living Cells
文献类型：期刊论文
| 作者 | Wu, Xiaofeng1,2; Shi, Wen1; Li, Xiaohua1; Ma, Huimin1,2 |
| 刊名 | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
 |
| 出版日期 | 2017-11-27 |
| 卷号 | 56期号:48页码:15319-15323 |
| 关键词 | Activity-based Probes Analytical Methods Fluorogenic Probes Molecular Recognition Monoamine Oxidase a |
| ISSN号 | 1433-7851 |
| DOI | 10.1002/anie.201708428 |
| 英文摘要 | Monoamine oxidase (MAO) has two isoforms, MAO-A and MAO-B, which show different functions, and thus selective fluorescence imaging is important for biological studies. Currently, however, specific detection of MAO-A remains a great challenge. Herein, we report a new strategy for specific imaging of MAO-A through the design of fluorogenic probes combining the characteristic structure of an inhibitor of the target enzyme along with propylamine as a recognition moiety. The high specificity of our representative probe is demonstrated by imaging MAO-A in different live cells such as SH-SY5Y (high levels of MAO-A) and HepG2 (high levels of MAO-B), and further validated by western blot analyses. The superior specificity of the probe may enable the accurate detection of MAO-A in complex biosystems. Importantly, the use of the characteristic structure of an inhibitor, as demonstrated in this work, may serve as a general strategy to design specific recognition moieties for fluorogenic probes for enzymes. |
| 语种 | 英语 |
| WOS记录号 | WOS:000416126600021 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/45053]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Ma, Huimin |
| 作者单位 | 1.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Key Lab Analyt Chem Living Biosyst, Inst Chem, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Xiaofeng,Shi, Wen,Li, Xiaohua,et al. A Strategy for Specific Fluorescence Imaging of Monoamine OxidaseA in Living Cells[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2017,56(48):15319-15323. |
| APA | Wu, Xiaofeng,Shi, Wen,Li, Xiaohua,&Ma, Huimin.(2017).A Strategy for Specific Fluorescence Imaging of Monoamine OxidaseA in Living Cells.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,56(48),15319-15323. |
| MLA | Wu, Xiaofeng,et al."A Strategy for Specific Fluorescence Imaging of Monoamine OxidaseA in Living Cells".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 56.48(2017):15319-15323. |
入库方式： OAI收割
来源：化学研究所
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