Single-molecule imaging and tracking of molecular dynamics in living cells
文献类型：期刊论文
| 作者 | Li, Nan1,2; Zhao, Rong1,2; Sun, Yahong1,2; Ye, Zi1,2; He, Kangmin3,4; Fang, Xiaohong1,2 |
| 刊名 | NATIONAL SCIENCE REVIEW
 |
| 出版日期 | 2017-09-01 |
| 卷号 | 4期号:5页码:739-760 |
| ISSN号 | 2095-5138 |
| 关键词 | Single-molecule Imaging Single-molecule Tracking Live-cell Imaging Fluorescence Microscopy Quantitative Analysis |
| DOI | 10.1093/nsr/nww055 |
| 英文摘要 | Unlike the ensemble-averaging measurements, the single-molecule imaging and tracking (SMIT) in living cells provides the real-time quantitative information about the locations, kinetics, dynamics and interactions of individual molecules in their native environments with high spatiotemporal resolution and minimal perturbation. The past decade has witnessed a transforming development in the methods of SMIT with living cells, including fluorescent probes, labeling strategies, fluorescence microscopy, and detection and tracking algorithms. In this review, we will discuss these aspects with a particular focus on their recent advancements. We will then describe representative single-molecule studies to illustrate how the single-molecule approaches can be applied to monitor biomolecular interaction/reaction dynamics, and extract the molecular mechanistic information for different cellular systems. |
| 语种 | 英语 |
| 出版者 | OXFORD UNIV PRESS |
| WOS记录号 | WOS:000418257300020 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/45219]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | He, Kangmin; Fang, Xiaohong |
| 作者单位 | 1.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Harvard Med Sch, Dept Cell Biol, Boston, MA 02115 USA 4.Boston Childrens Hosp, Cellular & Mol Med Program, Boston, MA 02115 USA |
| 推荐引用方式 GB/T 7714 | Li, Nan,Zhao, Rong,Sun, Yahong,et al. Single-molecule imaging and tracking of molecular dynamics in living cells[J]. NATIONAL SCIENCE REVIEW,2017,4(5):739-760. |
| APA | Li, Nan,Zhao, Rong,Sun, Yahong,Ye, Zi,He, Kangmin,&Fang, Xiaohong.(2017).Single-molecule imaging and tracking of molecular dynamics in living cells.NATIONAL SCIENCE REVIEW,4(5),739-760. |
| MLA | Li, Nan,et al."Single-molecule imaging and tracking of molecular dynamics in living cells".NATIONAL SCIENCE REVIEW 4.5(2017):739-760. |
入库方式： OAI收割
来源：化学研究所
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