Seamless Signal Transduction from Three-Dimensional Cultured Cells to a Superoxide Anions Biosensor via In Situ Self-Assembly of Dipeptide Hydrogel
文献类型：期刊论文
| 作者 | Lian, Meiling1; Xu, Liang1; Zhu, Xiaowen1; Chen, Xu1; Yang, Wensheng1; Wang, Tie2,3 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2017-12-05 |
| 卷号 | 89期号:23页码:12843-12849 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.7b03371 |
| 英文摘要 | This study demonstrates a new strategy for the development of a three-dimensional (3D) cell culture model-based cellular biosensing system. Distinctly different from the previously reported layering or separating fabrication of cell culture and sensing devices, herein living cells and enzymes as sensing elements are immobilized into a dipeptide-derived hydrogel matrix through simple one-pot self-assembly. The cells are then 3D cultured in the functional hydrogel, and the releasing superoxide anion (021 is detected in situ by a cascade superoxide dismutase and horseradish peroxidase-based electrochemical biosensor. This novel design provides considerable advantages, including the possibility of capturing molecular signals immediately after they are secreted from living cells, due to the close proximity of the enzymes and the O-2(center dot-)-producing cells. Furthermore, incorporating all components in a 3D matrix provides a confinement environment, that can lead to a concentrating effect of analysts. These properties allow the sensing device to achieve ultrahigh sensitivity and a precise response to a very low number of O-2(center dot-) molecules. The proposed approach, based on the self-assembly of a small molecular hydrogel, also simplifies experimental procedures and increases protocol flexibility to cell culture methodology and sensing design. Consequently, this novel 3D culture model-based cellular biosensing system is envisaged to be useful for cellular function and pathology, drug discovery, and toxicity studies. |
| 语种 | 英语 |
| WOS记录号 | WOS:000417549600034 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/45343]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Chen, Xu; Wang, Tie |
| 作者单位 | 1.Beijing Univ Chem Technol, State Key Lab Chem Resource Engn, Beijing 100029, Peoples R China 2.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lian, Meiling,Xu, Liang,Zhu, Xiaowen,et al. Seamless Signal Transduction from Three-Dimensional Cultured Cells to a Superoxide Anions Biosensor via In Situ Self-Assembly of Dipeptide Hydrogel[J]. ANALYTICAL CHEMISTRY,2017,89(23):12843-12849. |
| APA | Lian, Meiling,Xu, Liang,Zhu, Xiaowen,Chen, Xu,Yang, Wensheng,&Wang, Tie.(2017).Seamless Signal Transduction from Three-Dimensional Cultured Cells to a Superoxide Anions Biosensor via In Situ Self-Assembly of Dipeptide Hydrogel.ANALYTICAL CHEMISTRY,89(23),12843-12849. |
| MLA | Lian, Meiling,et al."Seamless Signal Transduction from Three-Dimensional Cultured Cells to a Superoxide Anions Biosensor via In Situ Self-Assembly of Dipeptide Hydrogel".ANALYTICAL CHEMISTRY 89.23(2017):12843-12849. |
入库方式： OAI收割
来源：化学研究所
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