Exploration of G-quadruplex function in c-Myb gene and its transcriptional regulation by topotecan
文献类型：期刊论文
| 作者 | Li, Fangyuan1; Zhou, Jiang1; Xu, Ming2; Yuan, Gu1 |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2018-02-01 |
| 卷号 | 107页码:1474-1479 |
| 关键词 | G-quadruplex C-myb Transcription Regulation |
| ISSN号 | 0141-8130 |
| DOI | 10.1016/j.ijbiomac.2017.10.010 |
| 英文摘要 | Our bioinformatics research shows that there are four G-rich sequences (S1-S4) in the upstream region of the transcription start site of c-Myb gene, and we have proved that these sequences have the ability to form G-quadruplex structures. This work mainly focuses on G-quadruplex function, recognition and transcription regulation in c-Myb gene, revealing a novel regulatory element in c-Myb proximal promoter region, and its transcription regulation by G-quadruplex binder. The research has identified that the enhancer effect in c-Myb transcription was primarily affected by the G-quadruplex formed by S1 sequence, and the up-regulation effect may due to the removal of repressive progress of MZF-1 by stabilizing G-quadruplex. Attentions were being paid to the development of G-quadruplex binders for selective recognition, and topotecan was found to have high binding affinity in vitro and could effectively affect the c-Myb transcription activities in cells. The regulation of G-quadruplex with binders in transcriptional, translational levels by Q-RT-PCR and western blot was in expectation of providing a strategy for gene expression modulation. In conclusion, our study revealed a G-quadruplex structure in c-Myb proximal promoter region, which was of great importance in the regulation of c-Myb function. (C) 2017 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000424062300012 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/45597]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhou, Jiang; Yuan, Gu |
| 作者单位 | 1.Peking Univ, Coll Chem & Mol Engn, Minist Educ,Dept Chem Biol, Beijing Natl Lab Mol Sci,Key Lab Bioorgan Chem &, Beijing 100871, Peoples R China 2.Peking Univ, Dept Cardiol,Minist Hlth, Inst Vasc Med,Key Lab Cardiovasc Mol Biol & Regul, Hosp 3,Key Lab Mol Cardiovasc Sci,Minist Educ, Beijing 100191, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Fangyuan,Zhou, Jiang,Xu, Ming,et al. Exploration of G-quadruplex function in c-Myb gene and its transcriptional regulation by topotecan[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2018,107:1474-1479. |
| APA | Li, Fangyuan,Zhou, Jiang,Xu, Ming,&Yuan, Gu.(2018).Exploration of G-quadruplex function in c-Myb gene and its transcriptional regulation by topotecan.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,107,1474-1479. |
| MLA | Li, Fangyuan,et al."Exploration of G-quadruplex function in c-Myb gene and its transcriptional regulation by topotecan".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 107(2018):1474-1479. |
入库方式： OAI收割
来源：化学研究所
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