Ethyl-substitutive Thioflavin T as a highly-specific fluorescence probe for detecting G-quadruplex structure
文献类型：期刊论文
| 作者 | Guan, Ai-Jiao1; Zhang, Xiu-Feng2; Sun, Xin2; Li, Qian1,3; Xiang, Jun-Feng1,3; Wang, Li-Xia1,3; Lan, Ling2,3; Yang, Feng-Min1,3; Xu, Shu-Juan1,3; Guo, Xiao-Meng1,3 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2018-02-08 |
| 卷号 | 8 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/s41598-018-20960-7 |
| 英文摘要 | G-quadruplex has attracted considerable attention due to their prevalent distribution in functional genomic regions and transcripts, which can importantly influence biological processes such as regulation of telomere maintenance, gene transcription and gene translation. Artificial receptor study has been developed for accurate identification of G-quadruplex from DNA species, since it is important for the G-quadruplex related basic research, clinical diagnosis, and therapy. Herein, fluorescent dye ThT-E, a derivative of the known fluorescence probe Thioflavin T (ThT), was designed and synthesized to effectively differentiate various G-quadruplex structures from other nucleic acid forms. Compared with methyl groups in ThT, three ethyl groups were introduced to ThT-E, which leads to strengthened affinity, selectivity and little inducing effect on the G-quadruplex formation. More importantly, ThT-E could be served as a visual tool to directly differentiate G-quadruplex solution even with naked eyes under illumination of ultraviolet light. Thus, this probe reported herein may hold great promise for high-throughput assay to screen G-quadruplex, which may widely apply to G-quadruplex-based potential diagnosis and therapy. |
| 语种 | 英语 |
| WOS记录号 | WOS:000424449100044 |
| 出版者 | NATURE PUBLISHING GROUP |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/45645]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Li, Qian; Wang, Li-Xia; Tang, Ya-Lin |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Struct Chem Unstable & Stable Speci, CAS Res Educ Ctr Excellence Mol Sci, Natl Lab Mol Sci,Ctr Mol Sci,Inst Chem, Beijing 100190, Peoples R China 2.North China Univ Sci & Technol, Coll Chem Engn, Tangshan 063009, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guan, Ai-Jiao,Zhang, Xiu-Feng,Sun, Xin,et al. Ethyl-substitutive Thioflavin T as a highly-specific fluorescence probe for detecting G-quadruplex structure[J]. SCIENTIFIC REPORTS,2018,8. |
| APA | Guan, Ai-Jiao.,Zhang, Xiu-Feng.,Sun, Xin.,Li, Qian.,Xiang, Jun-Feng.,...&Tang, Ya-Lin.(2018).Ethyl-substitutive Thioflavin T as a highly-specific fluorescence probe for detecting G-quadruplex structure.SCIENTIFIC REPORTS,8. |
| MLA | Guan, Ai-Jiao,et al."Ethyl-substitutive Thioflavin T as a highly-specific fluorescence probe for detecting G-quadruplex structure".SCIENTIFIC REPORTS 8(2018). |
入库方式： OAI收割
来源：化学研究所
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