Characterization of Hepatitis C Virus Core Protein Dimerization by Atomic Force Microscopy
文献类型：期刊论文
| 作者 | Li, Wenhui; Kou, Xiaolong; Xu, Jiachao; Zhou, Wei; Zhao, Rong; Zhang, Zhen; Fang, Xiaohong |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2018-04-03 |
| 卷号 | 90期号:7页码:4596-4602 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.7b05070 |
| 英文摘要 | Dimerization of core protein is a crucial step in the formation of the hepatitis C virus (HCV) nucleocapsid, and inhibition of dimer formation is regarded as an attractive approach to design anti-HCV drugs. In this work, we developed the atomic force microscopy based single molecular force spectroscopy (AFM-SMFS) method for the characterization of core protein dimerization with the advantages of small amount of sample consumption and no need of labeling. Interaction force of the core protein with its antibody or aptamer was analyzed to investigate its stoichiometry and binding property. The two specific binding forces were detected due to the probing of dimeric and monomeric core protein, respectively. Moreover, the binding property of protein dimer was different from the monomer. Our work offers a new approach to study the dimerization of core protein, as well as other proteins, and to screen the HCV candidate inhibitors. |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| WOS记录号 | WOS:000429385800050 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/46221]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Fang, Xiaohong |
| 作者单位 | Chinese Acad Sci, Inst Chem, CAS Res Educ Ctr Excellence Mol Sci, Key Lab Mol Nanostruct & Nanotechnol, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Wenhui,Kou, Xiaolong,Xu, Jiachao,et al. Characterization of Hepatitis C Virus Core Protein Dimerization by Atomic Force Microscopy[J]. ANALYTICAL CHEMISTRY,2018,90(7):4596-4602. |
| APA | Li, Wenhui.,Kou, Xiaolong.,Xu, Jiachao.,Zhou, Wei.,Zhao, Rong.,...&Fang, Xiaohong.(2018).Characterization of Hepatitis C Virus Core Protein Dimerization by Atomic Force Microscopy.ANALYTICAL CHEMISTRY,90(7),4596-4602. |
| MLA | Li, Wenhui,et al."Characterization of Hepatitis C Virus Core Protein Dimerization by Atomic Force Microscopy".ANALYTICAL CHEMISTRY 90.7(2018):4596-4602. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。