Atomic force microscopy study of the effect of HER 2 antibody on EGF mediated ErbB ligand-receptor interaction
文献类型：期刊论文
| 作者 | Zhang, Xuejie; Shi, Xiaoli; Xu, Li; Yuan, Jinghe; Fang, Xiaohong |
| 刊名 | NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
 |
| 出版日期 | 2013-07-01 |
| 卷号 | 9期号:5页码:627-635 |
| 关键词 | Atomic Force Microscopy (Afm) Single-molecule Force Spectroscopy (Smfs) Trastuzumab Pertuzumab Egfr/her2 Heterodimerization |
| ISSN号 | 1549-9634 |
| DOI | 10.1016/j.nano.2012.11.005 |
| 英文摘要 | HER2, a member of the epidermal growth factor receptor (ErbB) family, is over-expressed in many cancers. Trastuzumab and Pertuzumab are two monoclonal antibodies targeting different extracellular domains of HER2 for cancer therapy. As Pertuzumab binds to the dimerization arm of HER2, it can block HER2 heterodimerization and in turn ErbB signaling. Whether Trastuzumab has the same function is unclear. In this work, we have applied living-cell single-molecule force spectroscopy (SMFS) by Atomic Force Microscopy (AFM) to investigate the effect of Trastuzumab, as well as Pertuzumab, on HER2-modulated EGF-EGFR interaction. The results demonstrated that EGF bound to EGFR more stably in the cells co-expressing EGFR and HER2, and the binding enhancement in the presence of HER2 was inhibited by either Trastuzumab or Pertuzumab. Trastuzumab is expected to exert a similar inhibition effect on HER2/EGFR dimerization as Pertuzumab, although it does not bind directly to the dimerization arm of HER2. From the Clinical Editor: Living-cell single-molecule force spectroscopy (SMFS) combined by Atomic Force Microscopy (AFM) was used by this team of scientists to investigate the effect of two monoclonal antibodies used in cancer therapy, Trastuzumab and Pertuzumab, on HER2-modulated EGF-EGFR interaction, demonstrating the utility of this technique in characterizing the effects of protein-based therapeutics on membrane receptors. (C) 2013 Elsevier Inc. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000320593600005 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/46497]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Fang, Xiaohong |
| 作者单位 | Chinese Acad Sci, Beijing Natl Lab Mol Sci, CAS Key Lab Mol Nanostruct & Nanotechnol, Inst Chem, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Xuejie,Shi, Xiaoli,Xu, Li,et al. Atomic force microscopy study of the effect of HER 2 antibody on EGF mediated ErbB ligand-receptor interaction[J]. NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE,2013,9(5):627-635. |
| APA | Zhang, Xuejie,Shi, Xiaoli,Xu, Li,Yuan, Jinghe,&Fang, Xiaohong.(2013).Atomic force microscopy study of the effect of HER 2 antibody on EGF mediated ErbB ligand-receptor interaction.NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE,9(5),627-635. |
| MLA | Zhang, Xuejie,et al."Atomic force microscopy study of the effect of HER 2 antibody on EGF mediated ErbB ligand-receptor interaction".NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE 9.5(2013):627-635. |
入库方式： OAI收割
来源：化学研究所
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