Live-cell single-molecule imaging reveals clathrin and caveolin-1 dependent docking of SMAD4 at the cell membrane
文献类型：期刊论文
| 作者 | Yang, Yong1; Wolfram, Joy1,2; Shen, Jianliang1; Zhao, Yuliang2,3; Fang, Xiaohong4; Shen, Haifa1,5; Ferrari, Mauro1,6 |
| 刊名 | FEBS LETTERS
 |
| 出版日期 | 2013-12-11 |
| 卷号 | 587期号:24页码:3912-3920 |
| 关键词 | Smad4 Membrane Docking Single-molecule Fluorescence Imaging Clathrin Caveolin-1 |
| ISSN号 | 0014-5793 |
| DOI | 10.1016/j.febslet.2013.10.041 |
| 英文摘要 | Transforming growth factor beta (TGF-beta) signaling is important for many biological processes. Although the sequential events of this cascade are known, the dynamics remain speculative. Here, live-cell single-molecule total internal reflection fluorescence microscopy was used to monitor the dynamics of SMAD4, a TGF-beta downstream effector, in MDA-MB-231 breast cancer cells. Contrary to previous belief, SMAD4 was detectable at the cytoplasmic membrane, displaying two subpopulations with different membrane docking behaviors. These subpopulations were regulated by clathrin and caveolin-1, and had opposing roles in the nuclear shuttling of SMAD4 and the subsequent transcriptional regulation of genes associated with cell migration. The notion that membrane-docking behaviors of downstream molecules could predict the cellular response to growth factors may revolutionize the way we view cell signaling. |
| 语种 | 英语 |
| WOS记录号 | WOS:000327400100005 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/46499]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Yang, Yong |
| 作者单位 | 1.Houston Methodist Res Inst, Dept Nanomed, Houston, TX 77030 USA 2.Natl Ctr Nanosci & Technol China, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China 3.Chinese Acad Sci, Inst High Energy Phys, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing 100190, Peoples R China 5.Weill Cornell Med Coll, Dept Cell & Dev Biol, New York, NY 10065 USA 6.Weill Cornell Med Coll, Dept Med, New York, NY 10065 USA |
| 推荐引用方式 GB/T 7714 | Yang, Yong,Wolfram, Joy,Shen, Jianliang,et al. Live-cell single-molecule imaging reveals clathrin and caveolin-1 dependent docking of SMAD4 at the cell membrane[J]. FEBS LETTERS,2013,587(24):3912-3920. |
| APA | Yang, Yong.,Wolfram, Joy.,Shen, Jianliang.,Zhao, Yuliang.,Fang, Xiaohong.,...&Ferrari, Mauro.(2013).Live-cell single-molecule imaging reveals clathrin and caveolin-1 dependent docking of SMAD4 at the cell membrane.FEBS LETTERS,587(24),3912-3920. |
| MLA | Yang, Yong,et al."Live-cell single-molecule imaging reveals clathrin and caveolin-1 dependent docking of SMAD4 at the cell membrane".FEBS LETTERS 587.24(2013):3912-3920. |
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来源：化学研究所
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