Competitive Binding Sites of a Ruthenium Arene Anticancer Complex on Oligonucleotides Studied by Mass Spectrometry: Ladder-Sequencing versus Top-Down
文献类型：期刊论文
| 作者 | Wu, Kui1; Hu, Wenbing1; Luo, Qun1; Li, Xianchan1; Xiong, Shaoxiang1; Sadler, Peter J.2; Wang, Fuyi1 |
| 刊名 | JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
 |
| 出版日期 | 2013-03-01 |
| 卷号 | 24期号:3页码:410-420 |
| 关键词 | Mass Spectrometry Ladder-sequencing/top-down Ruthenium Anticancer Complexes Thymine/guanine Dna Oligonucleotide Binding Sites |
| ISSN号 | 1044-0305 |
| DOI | 10.1007/s13361-012-0539-z |
| 英文摘要 | We report identification of the binding sites for an organometallic ruthenium anticancer complex [(eta (6)-biphenyl)Ru(en)Cl][PF6] (1; en = ethylenediamine) on the 15-mer single-stranded oligodeoxynucleotides (ODNs), 5'-CTCTCTX(7)G(8)Y(9)CTTCTC-3' [X = Y = T (I); X = C and Y = A (II); X = A and Y = T (III); X = T and Y = A (IV)] by electrospray ionization mass spectrometry (ESI-MS) in conjunction with enzymatic digestion or tandem mass spectrometry (top-down MS). ESI-MS combined with enzymatic digestion (termed MS-based ladder-sequencing), is effective for identification of the thermodynamically-favored G-binding sites, but not applicable to determine the thermodynamically unstable T-binding sites because the T-bound adducts dissociate during enzymatic digestion. In contrast, top-down MS is efficient for localization of the T binding sites, but not suitable for mapping ruthenated G bases, due to the facile fragmentation of G bases from ODN backbones prior to the dissociation of the phosphodiester bonds. The combination of the two MS approaches reveals that G(8) in each ODN is the preferred binding site for 1, and that the T binding sites of 1 are either T-7 or T-11 on I and IV, and either T-6 or T-11 on II and III, respectively. These findings not only demonstrate for the first time that T-bases in single-stranded oligonucleotides are kinetically competitive with guanine for such organoruthenium complexes, but also illustrate the relative merits of the combination of ladder-sequencing and top-down MS approaches to elucidate the interactions of metal anticancer complexes with DNA. |
| 语种 | 英语 |
| WOS记录号 | WOS:000315515700013 |
| 出版者 | SPRINGER |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/46567]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Sadler, Peter J. |
| 作者单位 | 1.Chinese Acad Sci, Beijing Natl Lab Mol Sci, CAS Key Lab Analyt Chem Living Biosyst, Beijing Ctr Mass Spectrometry,Inst Chem, Beijing, Peoples R China 2.Univ Warwick, Dept Chem, Coventry CV4 7AL, W Midlands, England |
| 推荐引用方式 GB/T 7714 | Wu, Kui,Hu, Wenbing,Luo, Qun,et al. Competitive Binding Sites of a Ruthenium Arene Anticancer Complex on Oligonucleotides Studied by Mass Spectrometry: Ladder-Sequencing versus Top-Down[J]. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY,2013,24(3):410-420. |
| APA | Wu, Kui.,Hu, Wenbing.,Luo, Qun.,Li, Xianchan.,Xiong, Shaoxiang.,...&Wang, Fuyi.(2013).Competitive Binding Sites of a Ruthenium Arene Anticancer Complex on Oligonucleotides Studied by Mass Spectrometry: Ladder-Sequencing versus Top-Down.JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY,24(3),410-420. |
| MLA | Wu, Kui,et al."Competitive Binding Sites of a Ruthenium Arene Anticancer Complex on Oligonucleotides Studied by Mass Spectrometry: Ladder-Sequencing versus Top-Down".JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY 24.3(2013):410-420. |
入库方式： OAI收割
来源：化学研究所
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