Single-molecule monitoring in living cells by use of fluorescence microscopy
文献类型：期刊论文
| 作者 | Luo, Wangxi; He, Kangmin; Xia, Tie; Fang, Xiaohong |
| 刊名 | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
 |
| 出版日期 | 2013 |
| 卷号 | 405期号:1页码:43-49 |
| 关键词 | Single-molecule Imaging Fluorescence Microscopy Tirfm Living Cell |
| ISSN号 | 1618-2642 |
| DOI | 10.1007/s00216-012-6373-0 |
| 英文摘要 | Monitoring single molecules in living cells is becoming a powerful tool for study of the location, dynamics, and kinetics of individual biomolecules in real time. In recent decades, several optical imaging techniques, for example epi-fluorescence microscopy, total internal reflection fluorescence microscopy (TIRFM), confocal microscopy, quasi-TIRFM, and single-point edge excitation subdiffraction microscopy (SPEED), have been developed, and their capability of capturing single-molecule dynamics in living cells has been demonstrated. In this review, we briefly summarize recent advances in the use of these imaging techniques for monitoring single-molecules in living cells for a better understanding of important biological processes, and discuss future developments. |
| 语种 | 英语 |
| WOS记录号 | WOS:000313064000010 |
| 出版者 | SPRINGER HEIDELBERG |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/46593]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Fang, Xiaohong |
| 作者单位 | Chinese Acad Sci, Beijing Natl Lab Mol Sci, Key Lab Mol Nanostruct & Nanotechnol, Inst Chem, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Luo, Wangxi,He, Kangmin,Xia, Tie,et al. Single-molecule monitoring in living cells by use of fluorescence microscopy[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2013,405(1):43-49. |
| APA | Luo, Wangxi,He, Kangmin,Xia, Tie,&Fang, Xiaohong.(2013).Single-molecule monitoring in living cells by use of fluorescence microscopy.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,405(1),43-49. |
| MLA | Luo, Wangxi,et al."Single-molecule monitoring in living cells by use of fluorescence microscopy".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 405.1(2013):43-49. |
入库方式： OAI收割
来源：化学研究所
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