A tetra-layer microfluidic system for peptide affinity screening through integrated sample injection
文献类型：期刊论文
| 作者 | Wang, Weizhi; Huang, Yanyan; Jin, Yulong; Liu, Guoquan; Chen, Yi; Ma, Huimin; Zhao, Rui |
| 刊名 | ANALYST
 |
| 出版日期 | 2013 |
| 卷号 | 138期号:10页码:2890-2896 |
| ISSN号 | 0003-2654 |
| DOI | 10.1039/c3an00463e |
| 英文摘要 | A novel integrated microfluidic system was designed and fabricated for affinity peptide screening with in situ detection. A tetra-layer microfluidic hybrid chip containing two top eccentric diffluent layers, an inter-layer and a bottom screening layer, was developed as the core device of the system. The eccentric diffluent layers were ingeniously invented for the vertical sample delivery from 2 top-inlets into 12 bottom-inlets, which eliminated the use of excessive accessories and complicated pipelines currently used in other systems. By using six pH gradient generators, the magnetic bead-based screening in 36 parallel chambers was simultaneously carried out under 6 different pH conditions from 5.4 to 8.2. This allowed simultaneous screening of 6 compounds and each under 6 different pH conditions. The fabricated chip system was applied to screening of affinity peptides towards beta-endorphin antibody. The affinities of the peptide ligands to the antibody were assessed by on-chip confocal detection. The results from the screening study using this system indicated that the pentapeptide with the sequence of YGGFL had the highest affinity towards beta-endorphin antibody at pH 7.1, which was further confirmed by the ELISA assay using a 96-well plate format. This microfluidic screening system is automatic, low-cost and reusable for not only peptide screening but also other bioactive compounds screening towards target molecules. |
| 语种 | 英语 |
| WOS记录号 | WOS:000317867400017 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/46603]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhao, Rui |
| 作者单位 | Chinese Acad Sci, Beijing Natl Lab Mol Sci, Key Lab Analyt Chem Living Biosyst, Inst Chem, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Weizhi,Huang, Yanyan,Jin, Yulong,et al. A tetra-layer microfluidic system for peptide affinity screening through integrated sample injection[J]. ANALYST,2013,138(10):2890-2896. |
| APA | Wang, Weizhi.,Huang, Yanyan.,Jin, Yulong.,Liu, Guoquan.,Chen, Yi.,...&Zhao, Rui.(2013).A tetra-layer microfluidic system for peptide affinity screening through integrated sample injection.ANALYST,138(10),2890-2896. |
| MLA | Wang, Weizhi,et al."A tetra-layer microfluidic system for peptide affinity screening through integrated sample injection".ANALYST 138.10(2013):2890-2896. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。