Contribution of cholesterol moieties attached on MPEG-b-PCL-b-PLL to the cell uptake, endosomal escape and gene knockdown of the micelleplexes of siRNA
文献类型：期刊论文
| 作者 | Qi, Ruo-gu1,2; Wu, Su-hong1,2; Wang, Yu1; Chen, Jie1; Xie, Zhi-gang1; Huang, Yu-bin1; Jing, Xia-bin1 |
| 刊名 | CHINESE JOURNAL OF POLYMER SCIENCE
 |
| 出版日期 | 2013-06-01 |
| 卷号 | 31期号:6页码:912-923 |
| 关键词 | Amphiphilic Copolymer Cholesterol Endosome Escape Micelleplex Sirna Delivery |
| ISSN号 | 0256-7679 |
| DOI | 10.1007/s10118-013-1288-6 |
| 英文摘要 | To further enhance the transfection efficiency of a micelleplex system based on monomethoxy poly(ethylene glycol)-block-poly(E >-caprolactone)-block-poly(L-lysine) (MPEG-b-PCL-b-PLL), cholesterol (Chol) moieties are attached to the E >-termini of PLL segments to obtain MPEG-b-PCL-b-PLL/Chol. The structure and morphology of the copolymer are studied by H-1-NMR, TEM and DLS (dynamic light scattering). The cytotoxicity, cell uptake, endosomal release and mRNA knockdown are studied by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay, flow cytometry, CLSM (confocal laser scanning microscopy) and RT-PCR (real-time polymerase chain reaction). The results show that compared to their precursor MPEG-b-PCL-b-PLL, the cholesterol-grafted copolymer shows significantly lower toxicity, more rapid cellular endocytosis and endosome escape, and consequently displays enhanced siRNA transfection efficiency even at a lower N/P ratio. These improvements are ascribed to enhanced interaction of the cholesterol moieties with both cellular membrane and endosomal membrane. Moreover, effect of the PLL block length is examined. The final conclusion is that long enough PLL segments and incorporation of proper fraction of cholesterol onto the PLL segments benefit the enhancement of siRNA transfection efficiency. |
| 语种 | 英语 |
| WOS记录号 | WOS:000317985500008 |
| 出版者 | SPRINGER |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/46607]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Jing, Xia-bin |
| 作者单位 | 1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qi, Ruo-gu,Wu, Su-hong,Wang, Yu,et al. Contribution of cholesterol moieties attached on MPEG-b-PCL-b-PLL to the cell uptake, endosomal escape and gene knockdown of the micelleplexes of siRNA[J]. CHINESE JOURNAL OF POLYMER SCIENCE,2013,31(6):912-923. |
| APA | Qi, Ruo-gu.,Wu, Su-hong.,Wang, Yu.,Chen, Jie.,Xie, Zhi-gang.,...&Jing, Xia-bin.(2013).Contribution of cholesterol moieties attached on MPEG-b-PCL-b-PLL to the cell uptake, endosomal escape and gene knockdown of the micelleplexes of siRNA.CHINESE JOURNAL OF POLYMER SCIENCE,31(6),912-923. |
| MLA | Qi, Ruo-gu,et al."Contribution of cholesterol moieties attached on MPEG-b-PCL-b-PLL to the cell uptake, endosomal escape and gene knockdown of the micelleplexes of siRNA".CHINESE JOURNAL OF POLYMER SCIENCE 31.6(2013):912-923. |
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来源：化学研究所
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