Single-Molecule Force Spectroscopy Study on the Mechanism of RNA Disassembly in Tobacco Mosaic Virus
文献类型：期刊论文
| 作者 | Liu, Ningning1,2; Chen, Ying1; Peng, Bo3; Lin, Yuan3; Wang, Qian3,4; Su, Zhaohui3; Zhang, Wenke1; Li, Hongbin1,5; Shen, Jiacong1 |
| 刊名 | BIOPHYSICAL JOURNAL
 |
| 出版日期 | 2013-12-17 |
| 卷号 | 105期号:12页码:2790-2800 |
| ISSN号 | 0006-3495 |
| DOI | 10.1016/j.bpj.2013.10.005 |
| 英文摘要 | To explore the disassembly mechanism of tobacco mosaic virus (TMV), a model system for virus study, during infection, we have used single-molecule force spectroscopy to mimic and follow the process of RNA disassembly from the protein coat of TMV by the replisome (molecular motor) in vivo, under different pH and Ca-2+ concentrations. Dynamic force spectroscopy revealed the unbinding free-energy landscapes as that at pH 4.7 the disassembly process is dominated by one free-energy barrier, whereas at pH 7.0 the process is dominated by one barrier and that there exists a second barrier. The additional free-energy barrier at longer distance has been attributed to the hindrance of disordered loops within the inner channel of TMV, and the biological function of those protein loops was discussed. The combination of pH increase and Ca2+ concentration drop could weaken RNA-protein interactions so much that the molecular motor replisome would be able to pull and disassemble the rest of the genetic RNA from the protein coat in vivo. All these facts provide supporting evidence at the single-molecule level, to our knowledge for the first time, for the cotranslational disassembly mechanism during TMV infection under physiological conditions. |
| 语种 | 英语 |
| WOS记录号 | WOS:000328597400024 |
| 出版者 | CELL PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/46621]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Wang, Qian |
| 作者单位 | 1.Jilin Univ, State Key Lab Supramol Struct & Mat, Coll Chem, Changchun 130023, Peoples R China 2.Liaoning Shihua Univ, Sch Chem & Mat Sci, Fushun, Peoples R China 3.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China 4.Univ S Carolina, Dept Chem & Biochem, Columbia, SC 29208 USA 5.Univ British Columbia, Dept Chem, Vancouver, BC, Canada |
| 推荐引用方式 GB/T 7714 | Liu, Ningning,Chen, Ying,Peng, Bo,et al. Single-Molecule Force Spectroscopy Study on the Mechanism of RNA Disassembly in Tobacco Mosaic Virus[J]. BIOPHYSICAL JOURNAL,2013,105(12):2790-2800. |
| APA | Liu, Ningning.,Chen, Ying.,Peng, Bo.,Lin, Yuan.,Wang, Qian.,...&Shen, Jiacong.(2013).Single-Molecule Force Spectroscopy Study on the Mechanism of RNA Disassembly in Tobacco Mosaic Virus.BIOPHYSICAL JOURNAL,105(12),2790-2800. |
| MLA | Liu, Ningning,et al."Single-Molecule Force Spectroscopy Study on the Mechanism of RNA Disassembly in Tobacco Mosaic Virus".BIOPHYSICAL JOURNAL 105.12(2013):2790-2800. |
入库方式： OAI收割
来源：化学研究所
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