Single-Molecule Fluorescence Imaging in Living Cells
文献类型：期刊论文
| 作者 | Xia, Tie; Li, Nan; Fang, Xiaohong; Johnson, MA; Martinez, TJ |
| 刊名 | ANNUAL REVIEW OF PHYSICAL CHEMISTRY, VOL 64
 |
| 出版日期 | 2013 |
| 卷号 | 64页码:459-480 |
| 关键词 | Fluorescence Microscopy Stoichiometry Single-biomolecule Kinetics Mobility Superresolution Imaging |
| ISSN号 | 0066-426X |
| DOI | 10.1146/annurev-physchem-040412-110127 |
| 英文摘要 | The transition of single-molecule fluorescence detection and imaging from in vitro to living cells has greatly enriched our knowledge on the behavior of single biomolecules in their native environments and their roles in cellular processes. Here we review recent advances of single-molecule biophysical approaches to live-cell studies based on fluorescence imaging. We start by discussing the practical considerations in designing single-molecule fluorescence imaging in cells, including the choice of fluorescent probes, labeling methods, instrumentation, and imaging techniques. We then describe representative examples in detail to illustrate the physicochemical parameters that can be obtained by imaging individually labeled biomolecules in cells and what can be learned from such characterizations. |
| 语种 | 英语 |
| WOS记录号 | WOS:000321771600021 |
| 出版者 | ANNUAL REVIEWS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/46683]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Xia, Tie |
| 作者单位 | Chinese Acad Sci, Beijing Natl Lab Mol Sci, Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xia, Tie,Li, Nan,Fang, Xiaohong,et al. Single-Molecule Fluorescence Imaging in Living Cells[J]. ANNUAL REVIEW OF PHYSICAL CHEMISTRY, VOL 64,2013,64:459-480. |
| APA | Xia, Tie,Li, Nan,Fang, Xiaohong,Johnson, MA,&Martinez, TJ.(2013).Single-Molecule Fluorescence Imaging in Living Cells.ANNUAL REVIEW OF PHYSICAL CHEMISTRY, VOL 64,64,459-480. |
| MLA | Xia, Tie,et al."Single-Molecule Fluorescence Imaging in Living Cells".ANNUAL REVIEW OF PHYSICAL CHEMISTRY, VOL 64 64(2013):459-480. |
入库方式： OAI收割
来源：化学研究所
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