Poly(lactic-co-glycolic) Acid/Nanohydroxyapatite Scaffold Containing Chitosan Microspheres with Adrenomedullin Delivery for Modulation Activity of Osteoblasts and Vascular Endothelial Cells
文献类型：期刊论文
| 作者 | Wang, Lin1; Li, Chunyan2; Chen, Yingxin1; Dong, Shujun1; Chen, Xuesi3; Zhou, Yanmin2 |
| 刊名 | BIOMED RESEARCH INTERNATIONAL
 |
| 出版日期 | 2013 |
| ISSN号 | 2314-6133 |
| DOI | 10.1155/2013/530712 |
| 英文摘要 | Adrenomedullin (ADM) is a bioactive regulatory peptide that affects migration and proliferation of diverse cell types, including endothelial cells, smooth muscle cells, and osteoblast-like cells. This study investigated the effects of sustained release of ADM on the modulation activity of osteoblasts and vascular endothelial cells in vitro. Chitosan microspheres (CMs) were developed for ADM delivery. Poly(lactic-co-glycolic) acid and nano-hydroxyapatite were used to prepare scaffolds containing microspheres with ADM. The CMs showed rough surface morphology and high porosity, and they were well-distributed. The scaffolds exhibited relatively uniform pore sizes with interconnected pores. The addition of CMs improved the mechanical properties of the scaffolds without affecting their high porosity. In vitro degradation tests indicated that the addition of CMs increased the water absorption of the scaffolds and inhibited pH decline of phosphate-buffered saline medium. The expression levels of osteogenic-related and angiogenic-related genes were determined in MG63 cells and in human umbilical vein endothelial cells cultured on the scaffolds, respectively. The expression levels of osteogenic-related and angiogenic-related proteins were also detected by western blot analysis. Their expression levels in cells were improved on the ADM delivery scaffolds at a certain time point. The in vitro evaluation suggests that the microsphere-scaffold system is suitable as a model for bone tissue engineering. |
| 语种 | 英语 |
| WOS记录号 | WOS:000320800200001 |
| 出版者 | HINDAWI PUBLISHING CORPORATION |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/47035]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhou, Yanmin |
| 作者单位 | 1.Jilin Univ, Sch Stomatol, VIP Integrated Dept, Changchun 130021, Peoples R China 2.Jilin Univ, Sch Stomatol, Implant Ctr, Changchun 130021, Peoples R China 3.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Lin,Li, Chunyan,Chen, Yingxin,et al. Poly(lactic-co-glycolic) Acid/Nanohydroxyapatite Scaffold Containing Chitosan Microspheres with Adrenomedullin Delivery for Modulation Activity of Osteoblasts and Vascular Endothelial Cells[J]. BIOMED RESEARCH INTERNATIONAL,2013. |
| APA | Wang, Lin,Li, Chunyan,Chen, Yingxin,Dong, Shujun,Chen, Xuesi,&Zhou, Yanmin.(2013).Poly(lactic-co-glycolic) Acid/Nanohydroxyapatite Scaffold Containing Chitosan Microspheres with Adrenomedullin Delivery for Modulation Activity of Osteoblasts and Vascular Endothelial Cells.BIOMED RESEARCH INTERNATIONAL. |
| MLA | Wang, Lin,et al."Poly(lactic-co-glycolic) Acid/Nanohydroxyapatite Scaffold Containing Chitosan Microspheres with Adrenomedullin Delivery for Modulation Activity of Osteoblasts and Vascular Endothelial Cells".BIOMED RESEARCH INTERNATIONAL (2013). |
入库方式： OAI收割
来源：化学研究所
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