Fabrication of a Detection Platform with Boronic-Acid-Containing Zwitterionic Polymer Brush
文献类型：期刊论文
| 作者 | Song, Lingjie1,2; Zhao, Jie1; Luan, Shifang1; Ma, Jiao1; Liu, Jingchuan1; Xu, Xiaodong3,4; Yin, Jinghua1 |
| 刊名 | ACS APPLIED MATERIALS & INTERFACES
 |
| 出版日期 | 2013-12-25 |
| 卷号 | 5期号:24页码:13207-13215 |
| 关键词 | Atom Transfer Radical Polymerization (Atrp) Zwitterionic-based Materilals Boronic Acid (Ba) Antibody Immobilization Immunoassay |
| ISSN号 | 1944-8244 |
| DOI | 10.1021/am404206v |
| 英文摘要 | Development of technologies for biomedical detection platform is critical to meet the global challenges of various disease diagnoses, especially for point-of-use applications. Because of its natural simplicity, effectiveness, and easy repeatability, random covalent-binding technique is widely adopted in antibody immobilization. However, its antigen-binding capacity is relatively low when compared to site-specific immobilization of antibody. Herein, we report that a detection platform modified with boronic acid (BA)-containing sulfobetaine-based polymer brush. Mainly because of the advantage of oriented immobilization of antibody endowed with BA-containing three-dimensional polymer brush architecture, the platform had a high antigen-binding capacity. Notably, nonspecific protein adsorption was also suppressed by the zwitterionic pendants, thus greatly enhanced signal-to-noise (S/N) values for antigen recognition. Furthermore, antibodies captured by BA pendants could be released in dissociation media. This new platform is promising for potential applications in immunoassays. |
| 语种 | 英语 |
| WOS记录号 | WOS:000329137400059 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/47101]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Luan, Shifang |
| 作者单位 | 1.Acad Sinica, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Harbin Engn Univ, Coll Mat Sci & Chem Engn, Polymer Mat Res Ctr, Harbin 150001, Peoples R China 4.Harbin Engn Univ, Coll Mat Sci & Chem Engn, Minist Educ, Key Lab Superlight Mat & Surface Technol, Harbin 150001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, Lingjie,Zhao, Jie,Luan, Shifang,et al. Fabrication of a Detection Platform with Boronic-Acid-Containing Zwitterionic Polymer Brush[J]. ACS APPLIED MATERIALS & INTERFACES,2013,5(24):13207-13215. |
| APA | Song, Lingjie.,Zhao, Jie.,Luan, Shifang.,Ma, Jiao.,Liu, Jingchuan.,...&Yin, Jinghua.(2013).Fabrication of a Detection Platform with Boronic-Acid-Containing Zwitterionic Polymer Brush.ACS APPLIED MATERIALS & INTERFACES,5(24),13207-13215. |
| MLA | Song, Lingjie,et al."Fabrication of a Detection Platform with Boronic-Acid-Containing Zwitterionic Polymer Brush".ACS APPLIED MATERIALS & INTERFACES 5.24(2013):13207-13215. |
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来源：化学研究所
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