High-Salt-Tolerance Matrix for Facile Detection of Glucose in Rat Brain Microdialysates by MALDI Mass Spectrometry
文献类型：期刊论文
| 作者 | Chen, Rui1; Xu, Wenjun1; Xiong, Caiqiao1; Zhou, Xiaoyu1; Xiong, Shaoxiang1; Nie, Zongxiu1; Mao, Lanqun1; Chen, Yi1; Chang, Huan-Cheng2 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2012-01-03 |
| 卷号 | 84期号:1页码:465-469 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/ac202438a |
| 英文摘要 | Due to its strong ultraviolet absorption, high salt tolerance, and little interference in the low molecular weight region, N-(1-naphthyl) ethylenediamine dihydrochloride (NEDC) has been applied as a matrix to measure the level of glucose in rat brain microdialysates by matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) in combination with in vivo microdialysis. By monitoring the ion signals of (glucose + C1)(-) in the mass spectra, we achieved a low detection limit of similar to 10 mu M for glucose in 126 mM NaCl, which is a typical component in artificial cerebrospinal fluid, without prior sample purification. It is concluded that NEDC-assisted laser desorption/ionization (LDI) MS is a fast and general method for sensitive detection of small molecules (such as glucose and amino adds) in high ionic strength solutions. |
| 语种 | 英语 |
| WOS记录号 | WOS:000298763900066 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/47167]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Nie, Zongxiu |
| 作者单位 | 1.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Key Lab Analyt Chem Living Biosyst, Inst Chem, Beijing 100190, Peoples R China 2.Acad Sinica, Inst Atom & Mol Sci, Taipei 106, Taiwan |
| 推荐引用方式 GB/T 7714 | Chen, Rui,Xu, Wenjun,Xiong, Caiqiao,et al. High-Salt-Tolerance Matrix for Facile Detection of Glucose in Rat Brain Microdialysates by MALDI Mass Spectrometry[J]. ANALYTICAL CHEMISTRY,2012,84(1):465-469. |
| APA | Chen, Rui.,Xu, Wenjun.,Xiong, Caiqiao.,Zhou, Xiaoyu.,Xiong, Shaoxiang.,...&Chang, Huan-Cheng.(2012).High-Salt-Tolerance Matrix for Facile Detection of Glucose in Rat Brain Microdialysates by MALDI Mass Spectrometry.ANALYTICAL CHEMISTRY,84(1),465-469. |
| MLA | Chen, Rui,et al."High-Salt-Tolerance Matrix for Facile Detection of Glucose in Rat Brain Microdialysates by MALDI Mass Spectrometry".ANALYTICAL CHEMISTRY 84.1(2012):465-469. |
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来源：化学研究所
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