Protein photocrosslinking reveals dimer of dimers formation on MarR protein in Escherichia coli
文献类型：期刊论文
| 作者 | Chen Xing; Hao ZiYang; Chen, Peng R. |
| 刊名 | SCIENCE CHINA-CHEMISTRY
 |
| 出版日期 | 2012 |
| 卷号 | 55期号:1页码:106-111 |
| 关键词 | Marr Protein Antibiotic Resistance Protein Photocrosslinking Dna Binding Site Dimerization |
| ISSN号 | 1674-7291 |
| DOI | 10.1007/s11426-011-4437-1 |
| 英文摘要 | The multiple antibiotic resistance regulatory protein (MarR) binds to two promoter sites on the marO operator in Escherichia coli. Our study showed that more than one MarR dimer proteins bound to either of its two promoter sites (Site I and Site II), suggesting that MarR might form higher complexes than homodimers when bound to DNA inside E. coli cells. To further verify this hypothesis, we site-specifically incorporated a photocrosslinking probe at the interface between two MarR dimer proteins. Photolysis in living E. coli cells revealed a covalent linkage between the two interdimer subunits of MarR, suggesting that MarR forms dimer of dimers in vivo. |
| 语种 | 英语 |
| WOS记录号 | WOS:000298968200014 |
| 出版者 | SCIENCE PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/47169]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Chen, Peng R. |
| 作者单位 | Peking Univ, BNLMS, Dept Biol Chem, Coll Chem & Mol Engn, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen Xing,Hao ZiYang,Chen, Peng R.. Protein photocrosslinking reveals dimer of dimers formation on MarR protein in Escherichia coli[J]. SCIENCE CHINA-CHEMISTRY,2012,55(1):106-111. |
| APA | Chen Xing,Hao ZiYang,&Chen, Peng R..(2012).Protein photocrosslinking reveals dimer of dimers formation on MarR protein in Escherichia coli.SCIENCE CHINA-CHEMISTRY,55(1),106-111. |
| MLA | Chen Xing,et al."Protein photocrosslinking reveals dimer of dimers formation on MarR protein in Escherichia coli".SCIENCE CHINA-CHEMISTRY 55.1(2012):106-111. |
入库方式： OAI收割
来源：化学研究所
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