Biodegradable copolymers with identical cationic segments and their performance in siRNA delivery
文献类型：期刊论文
| 作者 | Qi, Ruogu1,2; Liu, Shi1,2; Chen, Jie1; Xiao, Haihua1,2; Yan, Lesan1,2; Huang, Yubin1; Jing, Xiabin1 |
| 刊名 | JOURNAL OF CONTROLLED RELEASE
 |
| 出版日期 | 2012-04-30 |
| 卷号 | 159期号:2页码:251-260 |
| 关键词 | Biodegradable Cationic Copolymers Click Chemistry Micelle-complex Pegylated Polyplexes Sirna Delivery |
| ISSN号 | 0168-3659 |
| DOI | 10.1016/j.jconrel.2012.01.015 |
| 英文摘要 | The biodegradable cationic copolymers monomethoxy poly(ethylene glycol)-block-poly(epsilon-caprolactone)-block-poly(L-lysine) (mPEG-b-PCL-b-PLL, further abbr. as "M") with a PCL block of different lengths and with a triazole linkage between the PCL and PLL blocks were synthesized via ring-opening polymerization of epsilon-caprolactone and L-lysine N-carboxyanhydride and click reactions. With the copolymer without the PCL block (mPEG-b-PLL, further abbr. as "P") as a control, self-assembling and complexation of these copolymers with siRNA were studied. siRNA-loading capacity, siRNA delivery performance and cellular uptake of the complexation products, namely complex from P (P-complex) and complexes from M1 and M2 (M1-complex and M2-complex), were further examined and correlated to their block-copolymer compositions. The results showed the block copolymers P, M1 and M2 have strong enough binding ability so that the complexes formed can resist the heparin displacement and RNase degradation. Compared to P-complex, M-complexes have smaller size and higher particle density so that they can be internalized via endocytosis more easily than free siRNA or P-complex and they display higher siRNA delivery efficiency and higher gene silencing efficiency than P-complex. The silencing efficiency of micellar complex is close to that of Lipofectamine (TM) 2000 and much better than PEI-25 kDa. Therefore, mPEG-b-PCL-b-PLL is expected to be a promising siRNA carrier. (C) 2012 Elsevier B.V. All rights reserved. GENE DELIVERY |
| 语种 | 英语 |
| WOS记录号 | WOS:000305001100012 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/47411]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Jing, Xiabin |
| 作者单位 | 1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qi, Ruogu,Liu, Shi,Chen, Jie,et al. Biodegradable copolymers with identical cationic segments and their performance in siRNA delivery[J]. JOURNAL OF CONTROLLED RELEASE,2012,159(2):251-260. |
| APA | Qi, Ruogu.,Liu, Shi.,Chen, Jie.,Xiao, Haihua.,Yan, Lesan.,...&Jing, Xiabin.(2012).Biodegradable copolymers with identical cationic segments and their performance in siRNA delivery.JOURNAL OF CONTROLLED RELEASE,159(2),251-260. |
| MLA | Qi, Ruogu,et al."Biodegradable copolymers with identical cationic segments and their performance in siRNA delivery".JOURNAL OF CONTROLLED RELEASE 159.2(2012):251-260. |
入库方式： OAI收割
来源：化学研究所
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