Measuring Binding Kinetics Of Surface-Bound Molecules Using The Surface Plasmon Resonance Technique
文献类型：期刊论文
| 作者 | Li BX(李宝霞) ; Chen J(陈娟); Long M(龙勉)
|
| 刊名 | Analytical Biochemistry
 |
| 出版日期 | 2008 |
| 页码 | 195-201 |
| 通讯作者邮箱 | mlong@imech.ac.cn |
| 关键词 | Cellular Off-Rate Cellular On-Rate Response Unit Kinetic Model Optical Biosensor Literature Selectin Antibody Biacore Antigen Constants Transport Protein Cells Shear |
| ISSN号 | 0003-2697 |
| 通讯作者 | Long, M (reprint author), Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100190, Peoples R China. |
| 中文摘要 | Surface plasmon resonance (SPR) technology and the Biacore biosensor have been widely used to measure the kinetics of biomolecular interactions in the fluid phase. In the past decade, the assay was further extended to measure reaction kinetics when two counterpart molecules are anchored on apposed surfaces. However, the cell binding kinetics has not been well quantified. Here we report development of a cellular kinetic model, combined with experimental procedures for cell binding kinetic measurements, to predict kinetic rates per cell. Human red blood cells coated with bovine serum albumin and anti-BSA monoclonal antibodies (mAbs) immobilized on the chip were used to conduct the measurements. Sensor-grams for BSA-coated RBC binding onto and debinding from the anti-BSA mAb-immobilized chip were obtained using a commercial Biacore 3000 biosensor, and analyzed with the cellular kinetic model developed. Not only did the model fit the data well, but it also predicted cellular on and off-rates as well as binding affinities from curve fitting. The dependence of flow duration, flow rate, and site density of BSA on binding kinetics was tested systematically, which further validated the feasibility and reliability of the new approach. Crown copyright (c) 2008 Published by Elsevier Inc. All rights reserved. |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | OPTICAL BIOSENSOR LITERATURE ; SELECTIN ; ANTIBODY ; BIACORE ; ANTIGEN ; CONSTANTS ; TRANSPORT ; PROTEIN ; CELLS ; SHEAR |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000255918000013 |
| 公开日期 | 2009-08-03 ; 2009-09-14 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/26038]  |
| 专题 | 力学研究所_力学所知识产出(1956-2008) |
| 推荐引用方式 GB/T 7714 | Li BX,Chen J,Long M. Measuring Binding Kinetics Of Surface-Bound Molecules Using The Surface Plasmon Resonance Technique[J]. Analytical Biochemistry,2008:195-201. |
| APA | 李宝霞,陈娟,&龙勉.(2008).Measuring Binding Kinetics Of Surface-Bound Molecules Using The Surface Plasmon Resonance Technique.Analytical Biochemistry,195-201. |
| MLA | 李宝霞,et al."Measuring Binding Kinetics Of Surface-Bound Molecules Using The Surface Plasmon Resonance Technique".Analytical Biochemistry (2008):195-201. |
入库方式： OAI收割
来源：力学研究所
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