Surface engineering of gold nanoparticles for in vitro siRNA delivery
文献类型：期刊论文
| 作者 | Zhao, Enyu1; Zhao, Zhixia1; Wang, Jiancheng1; Yang, Chunhui2; Chen, Chengjun1; Gao, Lingyan1; Feng, Qiang1; Hou, Wenjie1; Gao, Mingyuan2; Zhang, Qiang1 |
| 刊名 | NANOSCALE
 |
| 出版日期 | 2012 |
| 卷号 | 4期号:16页码:5102-5109 |
| ISSN号 | 2040-3364 |
| DOI | 10.1039/c2nr31290e |
| 英文摘要 | Cellular uptake, endosomal/lysosomal escape, and the effective dissociation from the carrier are a series of hurdles for specific genes to be delivered both in vitro and in vivo. To construct siRNA delivery systems, poly(allylamine hydrochloride) (PAH) and siRNA were alternately assembled on the surface of 11.8 +/- 0.9 nm Au nanoparticles (GNP), stabilized by denatured bovine serum albumin, by the ionic layer-by-layer (LbL) self-assembly method. By manipulating the outmost PAH layer, GNP-PAH vectors with different surface electric potentials were prepared. Then, the surface potential-dependent cytotoxicity of the resultant GNP-PAH particles was evaluated via sulforhodamine B (SRB) assay, while the surface potential-dependent cellular uptake efficiency was quantitatively analyzed by using the flow cytometry method based on carboxyfluorescein (FAM)-labeled siRNA. It was revealed that the GNP-PAH particles with surface potential of +25 mV exhibited the optimal cellular uptake efficiency and cytotoxicity for human breast cancer MCF-7 cells. Following these results, two more positively charged polyelectrolytes with different protonating abilities in comparison with PAH, i.e., polyethylenimine (PEI), and poly(diallyl dimethyl ammonium chloride) (PDDA), were chosen to fabricate similarly structured vectors. Confocal fluorescence microscopy studies indicated that siRNA delivered by GNP-PAH and GNP-PEI systems was better released than that delivered by the GNP-PDDA system. Further flow cytometric assays based on immunofluorescence staining of the epidermal growth factor receptor (EGFR) revealed that EGFR siRNA delivered by GNP-PAH and GNP-PEI exhibited similar down-regulation effects on EGFR expression in MCF-7 cells. The following dual fluorescence flow cytometry assays by co-staining phosphatidylserine and DNA suggested the EGFR siRNA delivered by GNP-PAH exhibited an improved silencing effect in comparison with that delivered by the commercial transfection reagent Lipofectamine 2000. |
| 语种 | 英语 |
| WOS记录号 | WOS:000306855500038 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/47469]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Wang, Jiancheng |
| 作者单位 | 1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Enyu,Zhao, Zhixia,Wang, Jiancheng,et al. Surface engineering of gold nanoparticles for in vitro siRNA delivery[J]. NANOSCALE,2012,4(16):5102-5109. |
| APA | Zhao, Enyu.,Zhao, Zhixia.,Wang, Jiancheng.,Yang, Chunhui.,Chen, Chengjun.,...&Zhang, Qiang.(2012).Surface engineering of gold nanoparticles for in vitro siRNA delivery.NANOSCALE,4(16),5102-5109. |
| MLA | Zhao, Enyu,et al."Surface engineering of gold nanoparticles for in vitro siRNA delivery".NANOSCALE 4.16(2012):5102-5109. |
入库方式： OAI收割
来源：化学研究所
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