Structure and stability of the complex formed by oligonucleotides
文献类型：期刊论文
| 作者 | Zheng, Cui1,2; Niu, Lin1,2; Yan, Jingjing1,2; Liu, Jie3; Luo, Ying3; Liang, Dehai1,2 |
| 刊名 | PHYSICAL CHEMISTRY CHEMICAL PHYSICS
 |
| 出版日期 | 2012 |
| 卷号 | 14期号:20页码:7352-7359 |
| ISSN号 | 1463-9076 |
| DOI | 10.1039/c2cp24086f |
| 英文摘要 | Polycations and cationic lipids have been widely used as non-viral vectors for the delivery of plasmid DNA, siRNA and anti-sense oligonucleotides. To demonstrate that one polycation can form a complex with several types of DNA, we conducted a comparative study on the complexation of poly(L-lysine) (PLL) with 2000 bp salmon testes DNA (dsDNA), 21 bp double-stranded oligonucleotides (ds-oligo), and 21 nt single-stranded oligonucleotides (ss-oligo) in PBS buffer. The complexes are prepared by a titration method and the process is monitored by laser light scattering. It was found that in most cases, ss-oligo and ds-oligo form complexes with higher molecular weights than the complex formed by dsDNA at the same +/- ratio immediately after mixing. More importantly, the complexes formed by oligonucleotides are not stable, the scattered intensity gradually decreases to the level of the solvent in weeks. Atomic force microscopy measurements also indicate that the freshly prepared complex is subject to environmental changes and could dissociate very quickly. The behaviour of oligonucleotides cannot be predicted by the classical polyelectrolyte theories. |
| 语种 | 英语 |
| WOS记录号 | WOS:000303444000020 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/47487]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Liang, Dehai |
| 作者单位 | 1.Peking Univ, Beijing Natl Lab Mol Sci, Coll Chem & Mol Engn, Beijing 100871, Peoples R China 2.Peking Univ, Key Lab Polymer Chem & Phys, Coll Chem & Mol Engn, Minist Educ, Beijing 100871, Peoples R China 3.Peking Univ, Dept Biomed Engn, Coll Engn, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng, Cui,Niu, Lin,Yan, Jingjing,et al. Structure and stability of the complex formed by oligonucleotides[J]. PHYSICAL CHEMISTRY CHEMICAL PHYSICS,2012,14(20):7352-7359. |
| APA | Zheng, Cui,Niu, Lin,Yan, Jingjing,Liu, Jie,Luo, Ying,&Liang, Dehai.(2012).Structure and stability of the complex formed by oligonucleotides.PHYSICAL CHEMISTRY CHEMICAL PHYSICS,14(20),7352-7359. |
| MLA | Zheng, Cui,et al."Structure and stability of the complex formed by oligonucleotides".PHYSICAL CHEMISTRY CHEMICAL PHYSICS 14.20(2012):7352-7359. |
入库方式： OAI收割
来源：化学研究所
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