Counting Bacteria Using Functionalized Gold Nanoparticles as the Light-Scattering Reporter
文献类型：期刊论文
| 作者 | Xu, Xiao1; Chen, Yang1; Wei, Hejia2,3; Xia, Bin1,2,3; Liu, Feng1; Li, Na1 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2012-11-20 |
| 卷号 | 84期号:22页码:9721-9728 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/ac302471c |
| 英文摘要 | A simple and rapid bacterial counting method was developed based on dark-field light-scattering imaging of bacteria and gold nanoparticle (AuNP) reporter simultaneously. Commercially available DH5 alpha E. coli strain was used as the model bacterium to demonstrate the feasibility of the proposed method. With antibody-conjugated AuNPs, the simple sample treatment and target E. coli strain recognition can be finished within 15-30 min, with a detection limit of 2-6 x 10(4) colony forming unit per milliliter (CFU/mL). By using 90 nm AuNPs as the light-scattering signal reporter, the bacterial recognition and counting can be easily performed with low-cost instrumentation such as an entry-level dark-field microscope setup and a common tungsten lamp as the light source. An automatic image analysis algorithm was also developed to facilitate robust and fast bacterial counting. The preliminary results of water, milk, and fruit juice sample analysis showed that this simple, fast, and cost-effective method can be easily adopted for routine bacterial detection. |
| 语种 | 英语 |
| WOS记录号 | WOS:000312434800012 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/47605]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Li, Na |
| 作者单位 | 1.Peking Univ, Key Lab Bioorgan Chem & Mol Engn, Coll Chem & Mol Engn, BNLMS,Minist Educ,Inst Analyt Chem, Beijing 100871, Peoples R China 2.Peking Univ, Beijing NMR Ctr, Beijing 100871, Peoples R China 3.Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Xiao,Chen, Yang,Wei, Hejia,et al. Counting Bacteria Using Functionalized Gold Nanoparticles as the Light-Scattering Reporter[J]. ANALYTICAL CHEMISTRY,2012,84(22):9721-9728. |
| APA | Xu, Xiao,Chen, Yang,Wei, Hejia,Xia, Bin,Liu, Feng,&Li, Na.(2012).Counting Bacteria Using Functionalized Gold Nanoparticles as the Light-Scattering Reporter.ANALYTICAL CHEMISTRY,84(22),9721-9728. |
| MLA | Xu, Xiao,et al."Counting Bacteria Using Functionalized Gold Nanoparticles as the Light-Scattering Reporter".ANALYTICAL CHEMISTRY 84.22(2012):9721-9728. |
入库方式： OAI收割
来源：化学研究所
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