Engineering a gold-specific regulon for cell-based visual detection and recovery of gold
文献类型：期刊论文
| 作者 | Wei, Wei1,2; Zhu, Tianze1; Wang, Yue2; Yang, Hualin2; Hao, Ziyang1; Chen, Peng R.1; Zhao, Jing2,3 |
| 刊名 | CHEMICAL SCIENCE
 |
| 出版日期 | 2012 |
| 卷号 | 3期号:6页码:1780-1784 |
| ISSN号 | 2041-6520 |
| DOI | 10.1039/c2sc01119k |
| 英文摘要 | A gold-specific sensory protein GolS from Samonella gol regulon was incorporated into E. coli, which in conjunction with an engineered downstream red fluorescence protein allowed the highly sensitive and selective whole-cell detection of gold(III) ions by naked eyes. The putative gold-chaperone, GolB, in the gol regulon was next verified to be specific to gold(I) ions over other metal ions including copper(I). The subsequent display of GolB on E. coli cell surface permitted selective enrichment of gold ions from media containing various thiophilic metal ions. The cell surface-enriched gold(I) was further shown to be easily recovered and the gold-deprived bacteria were capable for re-usage. E. coli bacteria harboring these gold-specific elements from the gol regulon could be a valuable tool for visual detection and facile recycling of gold ions from environmental resources. |
| 语种 | 英语 |
| WOS记录号 | WOS:000304365000005 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/47655]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Chen, Peng R. |
| 作者单位 | 1.Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China 2.Nanjing Univ, Sch Life Sci, State Key Lab Pharmaceut Biotechnol, Nanjing 210093, Jiangsu, Peoples R China 3.Peking Univ, Shenzhen Grad Sch, Nanomicro Mat Res Ctr, Sch Chem Biol & Biotechnol, Shenzhen 518055, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, Wei,Zhu, Tianze,Wang, Yue,et al. Engineering a gold-specific regulon for cell-based visual detection and recovery of gold[J]. CHEMICAL SCIENCE,2012,3(6):1780-1784. |
| APA | Wei, Wei.,Zhu, Tianze.,Wang, Yue.,Yang, Hualin.,Hao, Ziyang.,...&Zhao, Jing.(2012).Engineering a gold-specific regulon for cell-based visual detection and recovery of gold.CHEMICAL SCIENCE,3(6),1780-1784. |
| MLA | Wei, Wei,et al."Engineering a gold-specific regulon for cell-based visual detection and recovery of gold".CHEMICAL SCIENCE 3.6(2012):1780-1784. |
入库方式： OAI收割
来源：化学研究所
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