Fractal gold modified electrode for ultrasensitive thrombin detection
文献类型：期刊论文
| 作者 | Xu, Li-Ping1; Wang, Shuqi1; Dong, Haifeng1; Liu, Guodong3; Wen, Yongqiang1; Wang, Shutao2; Zhang, Xueji1 |
| 刊名 | NANOSCALE
 |
| 出版日期 | 2012 |
| 卷号 | 4期号:12页码:3786-3790 |
| ISSN号 | 2040-3364 |
| DOI | 10.1039/c2nr30826f |
| 英文摘要 | We report a label-free and ultrasensitive aptasensor based on a fractal gold modified (FracAu) electrode for thrombin detection with a femtomolar detection limit. The FracAu electrode was prepared by electrodeposition of hydrogen tetrachloroaurate (HAuCl4) onto a bare indium tin oxide (ITO) electrode surface. After this process the electrode was characterized by SEM. A thiol-modified aptamer against thrombin was immobilized on the FracAu electrode through a self-assembling process. Upon thrombin binding, the interfacial electron transfer of the FracAu electrode was perturbed by the formation of an aptamer-thrombin complex. The concentration of thrombin in the sample solution was determined by measuring the change in the oxidation peak current of hydroxymethyl ferrocene (C11H12FeO) with differential pulse voltammetry (DPV). The current response (reduced peak current) had a linear relationship with the logarithm of thrombin concentrations in the range of 10(-15) to 10(-10) M with a detection limit of 5.7 fM. Furthermore, the as-prepared FracAu electrode exhibited high selectivity. The application of FracAu electrodes may be extended to prepare other types of biosensors, such as immunosensors, enzyme biosensors and DNA biosensors. These results show that FracAu electrodes have great promise for clinical diagnosis of disease-related biomarkers. |
| 语种 | 英语 |
| WOS记录号 | WOS:000304666700032 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/47703]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Xu, Li-Ping |
| 作者单位 | 1.Univ Sci & Technol Beijing, Res Ctr Bioengn & Sensing Technol, Beijing 100083, Peoples R China 2.Chinese Acad Sci, BNLMS, Key Lab Organ Solids, Lab New Mat,Inst Chem, Beijing 100190, Peoples R China 3.N Dakota State Univ, Dept Chem & Biochem, Fargo, ND 58105 USA |
| 推荐引用方式 GB/T 7714 | Xu, Li-Ping,Wang, Shuqi,Dong, Haifeng,et al. Fractal gold modified electrode for ultrasensitive thrombin detection[J]. NANOSCALE,2012,4(12):3786-3790. |
| APA | Xu, Li-Ping.,Wang, Shuqi.,Dong, Haifeng.,Liu, Guodong.,Wen, Yongqiang.,...&Zhang, Xueji.(2012).Fractal gold modified electrode for ultrasensitive thrombin detection.NANOSCALE,4(12),3786-3790. |
| MLA | Xu, Li-Ping,et al."Fractal gold modified electrode for ultrasensitive thrombin detection".NANOSCALE 4.12(2012):3786-3790. |
入库方式： OAI收割
来源：化学研究所
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