A unique series of reversibly switchable fluorescent proteins with beneficial properties for various applications
文献类型：期刊论文
| 作者 | Chang, Hao1,2; Zhang, Mingshu1,5; Ji, Wei1; Chen, Juanjuan3; Zhang, Yongdeng4; Liu, Bei4; Lu, Jingze1; Zhang, Junlong3; Xu, Pingyong1; Xu, Tao1,4 |
| 刊名 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
 |
| 出版日期 | 2012-03-20 |
| 卷号 | 109期号:12页码:4455-4460 |
| ISSN号 | 0027-8424 |
| 关键词 | (f)Palm/storm Meos2 Mgeos Superresolution Microscopy |
| DOI | 10.1073/pnas.1113770109 |
| 英文摘要 | Reversibly switchable fluorescent proteins (RSFPs) have attracted widespread interest for emerging techniques including repeated tracking of protein behavior and superresolution microscopy. Among the limited number of RSFPs available, only Dronpa is widely employed for most cell biology applications due to its monomeric and other favorable photochemical properties. Here we developed a series of monomeric green RSFPs with beneficial optical characteristics such as high photon output per switch, high photostability, a broad range of switching rate, and pH-dependence, which make them potentially useful for various applications. One member of this series, mGeos-M, exhibits the highest photon budget and localization precision potential among all green RSFPs. We propose mGeos-M as a candidate to replace Dronpa for applications such as dynamic tracking, dual-color superresolution imaging, and optical lock-in detection. |
| 语种 | 英语 |
| 出版者 | NATL ACAD SCIENCES |
| WOS记录号 | WOS:000301712600028 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/47709]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Xu, Pingyong |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China 2.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China 3.Peking Univ, Coll Chem & Mol Engn, State Key Lab Rare Earth Mat Chem & Applicat, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China 4.Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Wuhan 430074, Peoples R China 5.Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chang, Hao,Zhang, Mingshu,Ji, Wei,et al. A unique series of reversibly switchable fluorescent proteins with beneficial properties for various applications[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2012,109(12):4455-4460. |
| APA | Chang, Hao.,Zhang, Mingshu.,Ji, Wei.,Chen, Juanjuan.,Zhang, Yongdeng.,...&Xu, Tao.(2012).A unique series of reversibly switchable fluorescent proteins with beneficial properties for various applications.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,109(12),4455-4460. |
| MLA | Chang, Hao,et al."A unique series of reversibly switchable fluorescent proteins with beneficial properties for various applications".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 109.12(2012):4455-4460. |
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来源：化学研究所
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