DNA-directed formation of peptide bond: a model study toward DNA-programmed peptide ligation
文献类型：期刊论文
| 作者 | Zhang, Chi; Li, Yizhou; Zhang, Mingda; Li, Xiaoyu |
| 刊名 | TETRAHEDRON
 |
| 出版日期 | 2012-07-01 |
| 卷号 | 68期号:26页码:5152-5156 |
| 关键词 | Dna-templated Synthesis Peptide Bond Formation Thiol-thioester Exchange Acyl Transfer Reaction Native Chemical Ligation |
| ISSN号 | 0040-4020 |
| DOI | 10.1016/j.tet.2012.04.032 |
| 英文摘要 | A model study of DNA-directed peptide ligation has been developed by transferring fluorescent reporting group from small molecule thioester to a DNA strand (template DNA) in the presence of a thiol-functionalized DNA strand (auxiliary DNA), mimicking the Native Chemical Ligation (NCL) reaction. This DNA-directed transfer shows dependence on the sequence complementarity of the two DNA strands, with in situ generated 4-thiolphenylmethyl functionalized oligonucleotide as the auxiliary DNA strand, under mild basic condition (pH=8.5), and with tris(2-carboxyethyl) phosphine hydrochloride (TCEP) as a reducing agent. Reactions with different amino acid alpha-thioesters resulted in varied transfer efficiencies from glycine to alpha-substituted amino acids. This study has provided the basic foundation to use DNA-programmed chemistry toward the chemical synthesis or unnatural modification of protein molecules. (C) 2012 Elsevier Ltd. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000305381700005 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/47747]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Li, Xiaoyu |
| 作者单位 | Peking Univ, BNLMS, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Inst Organ Chem,Coll Chem & Mol Engn, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Chi,Li, Yizhou,Zhang, Mingda,et al. DNA-directed formation of peptide bond: a model study toward DNA-programmed peptide ligation[J]. TETRAHEDRON,2012,68(26):5152-5156. |
| APA | Zhang, Chi,Li, Yizhou,Zhang, Mingda,&Li, Xiaoyu.(2012).DNA-directed formation of peptide bond: a model study toward DNA-programmed peptide ligation.TETRAHEDRON,68(26),5152-5156. |
| MLA | Zhang, Chi,et al."DNA-directed formation of peptide bond: a model study toward DNA-programmed peptide ligation".TETRAHEDRON 68.26(2012):5152-5156. |
入库方式： OAI收割
来源：化学研究所
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