Sequence Preference of alpha-Helix N-Terminal Tetrapeptide
文献类型：期刊论文
| 作者 | Qi, Yifei2; Liang, Huanhuan1; Han, Xinping1; Lai, Luhua1,2 |
| 刊名 | PROTEIN AND PEPTIDE LETTERS
 |
| 出版日期 | 2012-03-01 |
| 卷号 | 19期号:3页码:345-352 |
| 关键词 | Capping Box Helix Design Helix N-terminal N-cap Sequence Preference |
| ISSN号 | 0929-8665 |
| 英文摘要 | The alpha-helix is the most abundant secondary structure in proteins. Due to the specific i, i+4 hydrogen bond pattern, the two termini have unsatisfied hydrogen bonds, and are less constrained; in order to compensate for this, specific residues are preferred for the terminal positions. However, a nave combination of the statistically-preferred residues for each position may not result in a stable N-terminal helical sequence. In order to provide a set of preferable N-terminal peptides for -helix design, we have studied the N-terminal tetrapeptide sequence motifs that are favorable for helix formation using statistical analysis and atomistic simulations. A set of tetrapeptide sequences including TEEE and TPEE were found to be favorable motifs. In addition to forming more hydrogen bonds in the helical conformation, the favorable motifs also tended to form more capping boxes. To empirically test our predictions, we obtained 10 peptides with different N-terminal motifs and measured their alpha-helical content by circular dichroism spectroscopy. The experimental results agreed qualitatively with the statistical and simulation results. Furthermore, some of the suggested preferable tetrapeptide sequences have been successfully applied in de novo protein design. |
| 语种 | 英语 |
| WOS记录号 | WOS:000300702600012 |
| 出版者 | BENTHAM SCIENCE PUBL LTD |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/47817]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Lai, Luhua |
| 作者单位 | 1.Peking Univ, Coll Chem & Mol Engn, State Key Lab Struct Chem Unstable & Stable Speci, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China 2.Peking Univ, Ctr Theoret Biol, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qi, Yifei,Liang, Huanhuan,Han, Xinping,et al. Sequence Preference of alpha-Helix N-Terminal Tetrapeptide[J]. PROTEIN AND PEPTIDE LETTERS,2012,19(3):345-352. |
| APA | Qi, Yifei,Liang, Huanhuan,Han, Xinping,&Lai, Luhua.(2012).Sequence Preference of alpha-Helix N-Terminal Tetrapeptide.PROTEIN AND PEPTIDE LETTERS,19(3),345-352. |
| MLA | Qi, Yifei,et al."Sequence Preference of alpha-Helix N-Terminal Tetrapeptide".PROTEIN AND PEPTIDE LETTERS 19.3(2012):345-352. |
入库方式： OAI收割
来源：化学研究所
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