2,3,4,5-Tetrakis(3 ',4 '-dihydroxylphenyl)thiophene: A New Matrix for the Selective Analysis of Low Molecular Weight Amines and Direct Determination of Creatinine in Urine by MALDI-TOF MS
文献类型：期刊论文
| 作者 | Chen, Suming1; Chen, Li2; Wang, Jianing1; Hou, Jian1; He, Qing1; Liu, Jian'an3; Wang, Jiyun1; Xiong, Shaoxiang3; Yang, Guoqiang2; Nie, Zongxiu1,3 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2012-12-04 |
| 卷号 | 84期号:23页码:10291-10297 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/ac3021278 |
| 英文摘要 | Small organic matrixes are still the most commonly used ones in matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) because of their advantages of high sensitivity, convenience, and cost-effectiveness. However, due to the matrix interference in the low mass region, the direct analysis of low molecular weight amines in complex surroundings with conventional organic matrixes remains a challenge. Here, a new Bronsted-Lowry acid compound 2,3,4,5-tetrakis(3',4'-dihydroxylphenyl)-thiophene (DHPT) was designed, synthesized, and applied as a matrix for analysis of low molecular weight amines by MALDI-TOF MS. DHPT displays good selectivity in the analysis of amines without matrix-related interference and the low picomole/femtomole limit-of-detection was obtained in positive ion mode. With DHPT, the metabolites including creatinine, glycine, alloxan, allantoin, and 3-hydroxyhippuric acid in human urine were directly analyzed by MALDI-TOF MS. The identity of these metabolites was confirmed by tandem mass spectrometry. Furthermore, the urine creatinine was quantitatively determined using isotope-labeled internal standard. This DIPT-assisted LDI MS method provides a general approach for both qualitative and quantitative analysis of low molecular weight amines. |
| 语种 | 英语 |
| WOS记录号 | WOS:000311815300022 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/47887]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Nie, Zongxiu |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Key Lab Photochem, Beijing 100190, Peoples R China 3.Beijing Ctr Mass Spectrometry, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Suming,Chen, Li,Wang, Jianing,et al. 2,3,4,5-Tetrakis(3 ',4 '-dihydroxylphenyl)thiophene: A New Matrix for the Selective Analysis of Low Molecular Weight Amines and Direct Determination of Creatinine in Urine by MALDI-TOF MS[J]. ANALYTICAL CHEMISTRY,2012,84(23):10291-10297. |
| APA | Chen, Suming.,Chen, Li.,Wang, Jianing.,Hou, Jian.,He, Qing.,...&Nie, Zongxiu.(2012).2,3,4,5-Tetrakis(3 ',4 '-dihydroxylphenyl)thiophene: A New Matrix for the Selective Analysis of Low Molecular Weight Amines and Direct Determination of Creatinine in Urine by MALDI-TOF MS.ANALYTICAL CHEMISTRY,84(23),10291-10297. |
| MLA | Chen, Suming,et al."2,3,4,5-Tetrakis(3 ',4 '-dihydroxylphenyl)thiophene: A New Matrix for the Selective Analysis of Low Molecular Weight Amines and Direct Determination of Creatinine in Urine by MALDI-TOF MS".ANALYTICAL CHEMISTRY 84.23(2012):10291-10297. |
入库方式： OAI收割
来源：化学研究所
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