SiRNA Delivery Systems Based on Neutral Cross-Linked Dendrimers
文献类型：期刊论文
| 作者 | Liu, Jie1; Zhou, Jihan2,3; Luo, Ying1 |
| 刊名 | BIOCONJUGATE CHEMISTRY
 |
| 出版日期 | 2012-02-01 |
| 卷号 | 23期号:2页码:174-183 |
| ISSN号 | 1043-1802 |
| DOI | 10.1021/bc200433s |
| 英文摘要 | A neutral cross-linked dendritic system is described for use of delivering small interfering RNA (siRNA) for targeted gene silencing. By replacing the terminal amines with hydrazide groups and N-acetylgalactosamine (GalNAc) ligands, cationic polyamidoamine (PAMAM) dendrimers were transformed into neutral glycosylated carriers for siRNA delivery. Mainly owing to the pH sensitivity and the proton-absorption capability of the tertiary amines within the interior branches, these PAMAM derivatives showing neutrality under physiological conditions (pH 7.4) formed complexes with siRNA through electrostatic interactions at pH 5. Cross-linking procedures via reactions with glutaraldehyde were established, and cytocompatible cross linked systems loaded with siRNA obtained, with the particulate properties variable with the cross-linking condition and the GalNAc level in the dendritic carrier. In vitro cellular uptake and RNAi experiments showed that the cross-linked dendritic systems with an appropriate level of GalNAc composition effectively interacted with HepG2 cells and inhibited the expression of a luciferase reporter gene. Neutral cross-linked dendritic systems provide a new paradigm for designing siRNA delivery systems with biocompatibility and targeting capability. |
| 语种 | 英语 |
| WOS记录号 | WOS:000300213400004 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/47965]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Luo, Ying |
| 作者单位 | 1.Peking Univ, Coll Engn, Dept Biomed Engn, Beijing 100871, Peoples R China 2.Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, Minist Educ, Beijing 100871, Peoples R China 3.Peking Univ, Coll Chem & Mol Engn, Key Lab Polymer Chem & Phys, Minist Educ, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Jie,Zhou, Jihan,Luo, Ying. SiRNA Delivery Systems Based on Neutral Cross-Linked Dendrimers[J]. BIOCONJUGATE CHEMISTRY,2012,23(2):174-183. |
| APA | Liu, Jie,Zhou, Jihan,&Luo, Ying.(2012).SiRNA Delivery Systems Based on Neutral Cross-Linked Dendrimers.BIOCONJUGATE CHEMISTRY,23(2),174-183. |
| MLA | Liu, Jie,et al."SiRNA Delivery Systems Based on Neutral Cross-Linked Dendrimers".BIOCONJUGATE CHEMISTRY 23.2(2012):174-183. |
入库方式： OAI收割
来源：化学研究所
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