A label-free aptasensor for the sensitive and specific detection of cocaine using supramolecular aptamer fragments/target complex by electrochemical impedance spectroscopy
文献类型：期刊论文
| 作者 | Zhang, De-Wen1; Zhang, Fang-Ting1; Cui, Yi-Ran1; Deng, Qin-Pei1; Krause, Steffi2; Zhou, Ying-Lin1; Zhang, Xin-Xiang1 |
| 刊名 | TALANTA
 |
| 出版日期 | 2012-04-15 |
| 卷号 | 92页码:65-71 |
| 关键词 | Aptamer Fragment Cocaine Supramolecular Complex Label-free Electrochemical Impedance Spectroscopy |
| ISSN号 | 0039-9140 |
| DOI | 10.1016/j.talanta.2012.01.049 |
| 英文摘要 | A simple and label-free aptasensor for sensitive and specific detection of cocaine was developed by measuring the change in electrochemical impedance spectra (EIS), based on the formation of a supramolecular aptamer fragments/substrate complex. An anticocaine aptamer was divided into two fragments, Cx and Cy. Three different sensing interfaces, called Au/Cx5S/MCE, Au/Cy3S/MCE and Au/Cy5S/MCE, were fabricated by immobilizing Cx or Cy on a gold electrode through modifying their 5' or 3' end with a thiolated group followed by the treatment with mercaptoethanol (MCE). The formation of the corresponding supramolecular aptamer fragments/cocaine complex was investigated via monitoring electrochemical impedance spectra in the presence of [Fe(CN)(6)](3-/4-). The interfacial electron transfer resistance (R-et) was found to depend strongly on the cocaine concentration. Since the supramolecular aptamer fragments/cocaine complex was formed on the electrode surface, the sensing interface strongly affected the sensitivity of the aptasensor. Au/Cx5S/MCE was shown to have good sensitivity within a cocaine detection range of 0.1-20 mu M. Moreover, MCE was shown to improve the sensitivity of the aptasensor greatly. Even without the help of amplification or labeling, cocaine concentrations as low as 100 nM could be easily detected by the impedimetric aptasensor developed. The specificity and regeneration of the cocaine aptasensor were also investigated and satisfactory results were obtained. The developed aptasensor was successfully applied to detect the cocaine in biological fluids. (C) 2012 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000302500800008 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/48371]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhou, Ying-Lin |
| 作者单位 | 1.Peking Univ, Coll Chem, Key Lab Biochem & Mol Engn, BNLMS, Beijing 100871, Peoples R China 2.Univ London, Sch Engn & Mat Sci, London E1 4NS, England |
| 推荐引用方式 GB/T 7714 | Zhang, De-Wen,Zhang, Fang-Ting,Cui, Yi-Ran,et al. A label-free aptasensor for the sensitive and specific detection of cocaine using supramolecular aptamer fragments/target complex by electrochemical impedance spectroscopy[J]. TALANTA,2012,92:65-71. |
| APA | Zhang, De-Wen.,Zhang, Fang-Ting.,Cui, Yi-Ran.,Deng, Qin-Pei.,Krause, Steffi.,...&Zhang, Xin-Xiang.(2012).A label-free aptasensor for the sensitive and specific detection of cocaine using supramolecular aptamer fragments/target complex by electrochemical impedance spectroscopy.TALANTA,92,65-71. |
| MLA | Zhang, De-Wen,et al."A label-free aptasensor for the sensitive and specific detection of cocaine using supramolecular aptamer fragments/target complex by electrochemical impedance spectroscopy".TALANTA 92(2012):65-71. |
入库方式： OAI收割
来源：化学研究所
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