Cytotoxicity and TNF-alpha Secretion in RAW264.7 Macrophages Exposed to Different Fullerene Derivatives
文献类型：期刊论文
| 作者 | Xiang, Kun; Dou, Zengpei; Li, Yuankai; Xu, Yingying; Zhu, Jiadan; Yang, Shengtao; Sun, Hongfang; Liu, Yuanfang |
| 刊名 | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
 |
| 出版日期 | 2012-03-01 |
| 卷号 | 12期号:3页码:2169-2178 |
| 关键词 | Fullerene Derivatives Cytotoxicity Apoptosis Cellular Uptake Tnf-alpha Zeta Potential |
| ISSN号 | 1533-4880 |
| DOI | 10.1166/jnn.2012.5681 |
| 英文摘要 | Fullerene derivatives have been reported as potential nanomedicines, however the role of surface chemical modification on the biological effects remains unclear. In this study, five kinds of water soluble C-60 derivatives with different surface chemical modification, C-60-(OH)(20) (HFD), C-60-(beta-Ala)(10.1) (AFD), C-60-(Lys)(8.7) (KFD), C-60-(Arg)(8.6) (RFD) and C-60-(NH(CH2)(2)NH2)(8.8) (NFD) were synthesized. Their cytotoxicity as well as TNF-alpha secretions were evaluated in RAW264.7 macrophage cell line. The results show that no significant cytotoxicity can be observed upon 24 h exposure to C-60 derivatives at less than 50 mu g/mL. However, higher concentration (>100 mu g/mL) of these C-60 derivatives decreases the proliferation of RAW264.7. The cytotoxicity of these fullerene derivatives is probably through the apoptosis pathway, while the extent of cytotoxicity varies with the different surface charges. Higher celluar uptake of HFD was observed in RAW264.7 cells than AFD, which correlates with the more toxic effect of HFD over AFD. The secretion of cytokine tumor necrosis factor alpha (TNF-alpha) was determined to evaluate the immunostimulating activity of these fullerene derivatives. The data show that the fullerene derivatives with negative surface charges secrete more TNF-alpha, whereas derivatives with positive charges show insignificant effect. The possible influence of various surface charge property on the observed biological effects is discussed. |
| 语种 | 英语 |
| WOS记录号 | WOS:000305039700061 |
| 出版者 | AMER SCIENTIFIC PUBLISHERS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/48507]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Sun, Hongfang |
| 作者单位 | Peking Univ, Beijing Natl Lab Mol Sci, Dept Biol Chem, Coll Chem & Mol Engn, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiang, Kun,Dou, Zengpei,Li, Yuankai,et al. Cytotoxicity and TNF-alpha Secretion in RAW264.7 Macrophages Exposed to Different Fullerene Derivatives[J]. JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,2012,12(3):2169-2178. |
| APA | Xiang, Kun.,Dou, Zengpei.,Li, Yuankai.,Xu, Yingying.,Zhu, Jiadan.,...&Liu, Yuanfang.(2012).Cytotoxicity and TNF-alpha Secretion in RAW264.7 Macrophages Exposed to Different Fullerene Derivatives.JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,12(3),2169-2178. |
| MLA | Xiang, Kun,et al."Cytotoxicity and TNF-alpha Secretion in RAW264.7 Macrophages Exposed to Different Fullerene Derivatives".JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY 12.3(2012):2169-2178. |
入库方式： OAI收割
来源：化学研究所
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