Rapid and sensitive detection of auxins and flavonoids in plant samples by high-performance liquid chromatography coupled with tandem mass spectrometry
文献类型：期刊论文
| 作者 | Liang, Yuan; Zhu, Xiaocui; Wu, Tongbo; Zhao, Meiping; Liu, Huwei |
| 刊名 | JOURNAL OF SEPARATION SCIENCE
 |
| 出版日期 | 2012-10-01 |
| 卷号 | 35期号:19页码:2559-2566 |
| 关键词 | Indole-3-acetic Acid Kaempferol Liquid Chromatography-quadrupole Time-of-flight Tandem Mass Spectrometry Methyl Indole-3-acetic Acid Ester Quercetin |
| ISSN号 | 1615-9306 |
| DOI | 10.1002/jssc.201200418 |
| 英文摘要 | Simultaneous determination of indole-3-acetic acid and methyl indole-3-acetic acid ester in small amounts of plant tissue is essential for elucidating their mutual transformation mechanism and the in vivo function of methyl indole-3-acetic acid ester. Rapid quantification of flavonoids in the same sample is important for clarifying their roles in the transport of auxins and other phytohormones. Herein, we describe a simple method for the simultaneous determination of indole-3-acetic acid and its methyl ester in the roots of the Arabidopsis thaliana seedlings and a protocol for the rapid extraction and quantification of quercetin and kaempferol in these seedlings. High-performance liquid chromatography coupled with electrospray ionization time-of-flight tandem mass spectrometry was used for the detection of all the compounds. Negative data for indole-3-acetic acid and positive data for methyl indole-3-acetic acid ester were collected in two successive files with a single injection of the extracted sample. Under optimized conditions, the limit of detection for the four compounds was 2 ng/mL for indole-3-acetic acid, 0.5 ng/mL for methyl indole-3-acetic acid ester, 5 ng/mL for quercetin, and 1 ng/mL for kaempferol, respectively. Because of the high sensitivity of the assay, only 210 mg of the plant material was required to obtain quantitative results. |
| 语种 | 英语 |
| WOS记录号 | WOS:000309059800006 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/48705]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhao, Meiping |
| 作者单位 | Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, MOE Key Lab Bioorgan Chem & Mol Engn, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liang, Yuan,Zhu, Xiaocui,Wu, Tongbo,et al. Rapid and sensitive detection of auxins and flavonoids in plant samples by high-performance liquid chromatography coupled with tandem mass spectrometry[J]. JOURNAL OF SEPARATION SCIENCE,2012,35(19):2559-2566. |
| APA | Liang, Yuan,Zhu, Xiaocui,Wu, Tongbo,Zhao, Meiping,&Liu, Huwei.(2012).Rapid and sensitive detection of auxins and flavonoids in plant samples by high-performance liquid chromatography coupled with tandem mass spectrometry.JOURNAL OF SEPARATION SCIENCE,35(19),2559-2566. |
| MLA | Liang, Yuan,et al."Rapid and sensitive detection of auxins and flavonoids in plant samples by high-performance liquid chromatography coupled with tandem mass spectrometry".JOURNAL OF SEPARATION SCIENCE 35.19(2012):2559-2566. |
入库方式： OAI收割
来源：化学研究所
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