A CE-LIF method to monitor autophagy by directly detecting LC3 proteins in HeLa cells
文献类型：期刊论文
| 作者 | Jin, Yue1; Chen, Cheng1; Meng, Lingchen1; Chen, Jitao1; Li, Meixian1; Zhu, Zhiwei1; Lin, Jian2 |
| 刊名 | ANALYST
 |
| 出版日期 | 2012 |
| 卷号 | 137期号:23页码:5571-5575 |
| ISSN号 | 0003-2654 |
| DOI | 10.1039/c2an36011j |
| 英文摘要 | A fast, simple and quantitative approach was established for monitoring autophagy in HeLa cells by directly detecting the conversion of green fluorescent protein (GFP) labelled autophagy markers, GFP-LC3-I to GFP-LC3-II, in crude cellular extract using capillary electrophoresis (CE) with laser-induced fluorescence (LIF). Compared with the traditional methods, this proposed method is simpler and more reliable. Moreover, high sensitivity, with a limit of detection (LOD) of 0.48 ppt (bovine serum albumin as standard protein), was obtained by an in-capillary derivatization method coupled with a field-enhanced sample injection (FESI) technique, this may allow the success of this technique in the detection of endogenous markers of autophagy in cells. |
| 语种 | 英语 |
| WOS记录号 | WOS:000310375200018 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/48775]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhu, Zhiwei |
| 作者单位 | 1.Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China 2.Peking Univ, Beijing Nucl Magnet Resonance Ctr, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jin, Yue,Chen, Cheng,Meng, Lingchen,et al. A CE-LIF method to monitor autophagy by directly detecting LC3 proteins in HeLa cells[J]. ANALYST,2012,137(23):5571-5575. |
| APA | Jin, Yue.,Chen, Cheng.,Meng, Lingchen.,Chen, Jitao.,Li, Meixian.,...&Lin, Jian.(2012).A CE-LIF method to monitor autophagy by directly detecting LC3 proteins in HeLa cells.ANALYST,137(23),5571-5575. |
| MLA | Jin, Yue,et al."A CE-LIF method to monitor autophagy by directly detecting LC3 proteins in HeLa cells".ANALYST 137.23(2012):5571-5575. |
入库方式： OAI收割
来源：化学研究所
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