One-Pot Synthesis of Polypeptide-Gold Nanoconjugates for in Vitro Gene Transfection
文献类型：期刊论文
| 作者 | Yan, Xuehai1; Blacklock, Jenifer1; Li, Junbai2; Moehwald, Helmuth1 |
| 刊名 | ACS NANO
 |
| 出版日期 | 2012 |
| 卷号 | 6期号:1页码:111-117 |
| 关键词 | Peptide Gold Nanoparticle One-pot Synthesis Gene Transfection Nanoconjugate Delivery Vector |
| ISSN号 | 1936-0851 |
| DOI | 10.1021/nn202939s |
| 英文摘要 | We present a general strategy to create polypeptide-gold nanoconjugates by a one-pot synthesis approach, where polypeptides act not only as capping agents but also as reductants for the formation of gold nanoparticles without the need of an additional reducing agent. The present approach is environmentally benign, facile, and flexible for the design of functional polypeptide-gold nanoconjugates. As a demonstration of as-synthesized nanoconjugates for biomedical applications, the resulting positively charged polypeptide-conjugated gold nanoparticles are applied for gene delivery. A gradual and prolonged intracellular uptake and transfection is achieved, and transfection activity Is maintained for almost two weeks with no obvious cytotoxicity. The biologically based method presented In this work will provide a new alternative in creating a variety of multifunctional polypeptide-metallic nanoconjugates In a simple and straightforward manner, which will be more advantageous for their applications in biomedicine. |
| 语种 | 英语 |
| WOS记录号 | WOS:000299368300014 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/48781]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Yan, Xuehai |
| 作者单位 | 1.Max Planck Inst Colloids & Interfaces, D-14476 Potsdam, Germany 2.Chinese Acad Sci, Key Lab Colloid & Interface Sci, BNLMS, Inst Chem, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yan, Xuehai,Blacklock, Jenifer,Li, Junbai,et al. One-Pot Synthesis of Polypeptide-Gold Nanoconjugates for in Vitro Gene Transfection[J]. ACS NANO,2012,6(1):111-117. |
| APA | Yan, Xuehai,Blacklock, Jenifer,Li, Junbai,&Moehwald, Helmuth.(2012).One-Pot Synthesis of Polypeptide-Gold Nanoconjugates for in Vitro Gene Transfection.ACS NANO,6(1),111-117. |
| MLA | Yan, Xuehai,et al."One-Pot Synthesis of Polypeptide-Gold Nanoconjugates for in Vitro Gene Transfection".ACS NANO 6.1(2012):111-117. |
入库方式： OAI收割
来源：化学研究所
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