Rapid Flu Diagnosis Using Silicon Nanowire Sensor
文献类型：期刊论文
| 作者 | Shen, Fangxia1; Wang, Jindong2,3; Xu, Zhenqiang1; Wu, Yan1; Chen, Qi1; Li, Xiaoguang4; Jie, Xu4; Li, Lidong3; Yao, Maosheng1; Guo, Xuefeng2,5 |
| 刊名 | NANO LETTERS
 |
| 出版日期 | 2012-07-01 |
| 卷号 | 12期号:7页码:3722-3730 |
| 关键词 | Influenza a H3n2 Virus Exhaled Breath Condensate Silicon Nanowire Flu Diagnosis Rt-qpcr |
| ISSN号 | 1530-6984 |
| DOI | 10.1021/nl301516z |
| 英文摘要 | Influenza epidemics worldwide result in substantial economic and human costs annually. However, rapid and reliable flu diagnosis methods are significantly lacking. Here we have demonstrated the selective detection of influenza A viruses down to 29 viruses/mu L in clinical exhaled breath condensate (EBC) samples (diluted by 100-fold) within minutes using silicon nanowire (SiNW) sensor devices. For 90% of the cases, we have observed that EBC samples tested positive or negative by gold standard method RT-qPCR generated corresponding positive or negative SiNW sensor responses. High selectivity of SiNW sensing was also demonstrated using H1N1 viruses, 8 iso PGF 2a, and inert nanoparticles. Finally, magnetic beads were shown capable of enhancing SiNW sensing directly for low level viruses and 8 iso PGF 2a. When calibrated, by virus standards and EBC controls, our work suggests that the SiNW sensor device can be reliably applied to the diagnosis of flu in a clinical setting with 2 orders of magnitude less time compared to the gold standard method RT-qPCR. |
| 语种 | 英语 |
| WOS记录号 | WOS:000306296200062 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/48861]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Yao, Maosheng |
| 作者单位 | 1.Peking Univ, Coll Environm Sci & Engn, State Key Joint Lab Environm Simulat & Pollut Con, Beijing 100871, Peoples R China 2.Peking Univ, Coll Chem & Mol Engn, State Key Lab Struct Chem Unstable & Stable Speci, Ctr Nanochem,Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China 3.Univ Sci & Technol Beijing, Sch Mat Sci & Engn, Beijing 100083, Peoples R China 4.Peking Univ, Hosp 3, Dept Infect Dis, Beijing 100191, Peoples R China 5.Peking Univ, Coll Engn, Dept Adv Mat & Nanotechnol, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shen, Fangxia,Wang, Jindong,Xu, Zhenqiang,et al. Rapid Flu Diagnosis Using Silicon Nanowire Sensor[J]. NANO LETTERS,2012,12(7):3722-3730. |
| APA | Shen, Fangxia.,Wang, Jindong.,Xu, Zhenqiang.,Wu, Yan.,Chen, Qi.,...&Zhu, Tong.(2012).Rapid Flu Diagnosis Using Silicon Nanowire Sensor.NANO LETTERS,12(7),3722-3730. |
| MLA | Shen, Fangxia,et al."Rapid Flu Diagnosis Using Silicon Nanowire Sensor".NANO LETTERS 12.7(2012):3722-3730. |
入库方式： OAI收割
来源：化学研究所
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