PEGylated Graphene Oxide-Mediated Protein Delivery for Cell Function Regulation
文献类型：期刊论文
| 作者 | Shen, He1,3,4; Liu, Min1; He, Huixin2; Zhang, Liming1; Huang, Jie1; Chong, Yu1; Dai, Jianwu1; Zhang, Zhijun1 |
| 刊名 | ACS APPLIED MATERIALS & INTERFACES
 |
| 出版日期 | 2012-11-01 |
| 卷号 | 4期号:11页码:6317-6323 |
| 关键词 | Graphene Oxide Loading Capacity Protein Delivery Cellular Uptake Enzymatic Hydrolysis Regulation Of Cell Function |
| ISSN号 | 1944-8244 |
| DOI | 10.1021/am3019367 |
| 英文摘要 | Delivery of proteins into cells may alter cellular functions as various proteins are involved in cellular signaling by activating or deactivating the corresponding pathways and, therefore, can be used in cancer therapy. In this study, we have demonstrated for the first time that PEGylated graphene oxide (GO) can be exploited as a nanovector for efficient delivery of proteins into cells. In this approach, GO was functionalized with amine-terminated 6-armed polyethylene glycol (PEG) molecules, thereby providing GO with proper physiological stability and biocompatibility. Proteins were then loaded onto PEG-grafted GO (GO-PEG) with high payload via noncovalent interactions. GO-PEG could deliver proteins to cytoplasm efficiently, protecting them from enzymatic hydrolysis. The protein delivered by GO-PEG reserves its biological activity that regulates the cell fate. As a result, delivery of ribonuclease A (RNase A) led to cell death and transport of protein kinase A (PKA) induced cell growth. Taken together, this work demonstrated the feasibility of PEGlyated GO as a promising protein delivery vector with high biocompatibility, high payload capacity and, more importantly, capabilities of protecting proteins from enzymatic hydrolysis and retaining their biological functions. |
| 语种 | 英语 |
| WOS记录号 | WOS:000311521900080 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/48947]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhang, Zhijun |
| 作者单位 | 1.Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Div Nanobiomed, Suzhou 215123, Peoples R China 2.Rutgers State Univ, Dept Chem, Newark, NJ 07102 USA 3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 4.Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shen, He,Liu, Min,He, Huixin,et al. PEGylated Graphene Oxide-Mediated Protein Delivery for Cell Function Regulation[J]. ACS APPLIED MATERIALS & INTERFACES,2012,4(11):6317-6323. |
| APA | Shen, He.,Liu, Min.,He, Huixin.,Zhang, Liming.,Huang, Jie.,...&Zhang, Zhijun.(2012).PEGylated Graphene Oxide-Mediated Protein Delivery for Cell Function Regulation.ACS APPLIED MATERIALS & INTERFACES,4(11),6317-6323. |
| MLA | Shen, He,et al."PEGylated Graphene Oxide-Mediated Protein Delivery for Cell Function Regulation".ACS APPLIED MATERIALS & INTERFACES 4.11(2012):6317-6323. |
入库方式： OAI收割
来源：化学研究所
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