A new continuous fluorometric assay for acetylcholinesterase activity and inhibitor screening with emissive core-shell silica particles containing tetraphenylethylene fluorophore
文献类型：期刊论文
| 作者 | Shen, Xiang; Liang, Fuxin; Zhang, Guanxin; Zhang, Deqing |
| 刊名 | ANALYST
 |
| 出版日期 | 2012 |
| 卷号 | 137期号:9页码:2119-2123 |
| ISSN号 | 0003-2654 |
| DOI | 10.1039/c2an35154d |
| 英文摘要 | Emissive core-shell silica particles with tetraphenylethylene moieties were prepared and characterized. Fluorescence quenching was observed for the silica particles upon addition of compound 2 (Dabcyl-ACh). This was attributed to the electrostatic interaction between the silica particles and 2 and the resulting photoinduced energy transfer between them. After incubation with AChE, the fluorescence intensity started to increase. The fluorescence enhancement became more significant when the concentration of AChE was higher. The reaction kinetic parameters for AChE were successfully estimated with the silica particles and 2. These results reveal that the ensemble of the silica particles and 2 can be utilized for AChE assay. Moreover, the fluorescence spectra of the ensemble of the silica particles and 2 containing AChE were also measured after further addition of either neostigmine or tacrine which are typical inhibitors of AChE. The results manifest that the ensemble of the emissive silica particles and 2 is also useful for screening the inhibitors of AChE. |
| 语种 | 英语 |
| WOS记录号 | WOS:000302308600023 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/49047]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhang, Guanxin |
| 作者单位 | Chinese Acad Sci, Inst Chem, Organ Solids Lab, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shen, Xiang,Liang, Fuxin,Zhang, Guanxin,et al. A new continuous fluorometric assay for acetylcholinesterase activity and inhibitor screening with emissive core-shell silica particles containing tetraphenylethylene fluorophore[J]. ANALYST,2012,137(9):2119-2123. |
| APA | Shen, Xiang,Liang, Fuxin,Zhang, Guanxin,&Zhang, Deqing.(2012).A new continuous fluorometric assay for acetylcholinesterase activity and inhibitor screening with emissive core-shell silica particles containing tetraphenylethylene fluorophore.ANALYST,137(9),2119-2123. |
| MLA | Shen, Xiang,et al."A new continuous fluorometric assay for acetylcholinesterase activity and inhibitor screening with emissive core-shell silica particles containing tetraphenylethylene fluorophore".ANALYST 137.9(2012):2119-2123. |
入库方式： OAI收割
来源：化学研究所
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