A universal mismatch-directed signal amplification platform for ultra-selective and sensitive DNA detection under mild isothermal conditions
文献类型：期刊论文
| 作者 | Xiao, Xianjin; Zhang, Chen; Su, Xin; Song, Chen; Zhao, Meiping |
| 刊名 | CHEMICAL SCIENCE
 |
| 出版日期 | 2012 |
| 卷号 | 3期号:7页码:2257-2261 |
| ISSN号 | 2041-6520 |
| DOI | 10.1039/c2sc20189e |
| 英文摘要 | We describe a novel mismatch-directed signal amplification method which can be performed easily under mild isothermal conditions for the ultra-selective and sensitive detection of any DNA sequence of interest. By taking advantage of the unique discrimination properties of an endonuclease IV and lambda exonuclease coupling system in cleavage reactions of DNA duplexes containing apurinic/apyrimidinic sites with different adjacent mismatched bases, the established assay enables sensitive detection of 1.0 fmol of target strand and selective differentiation of 0.5% target strand from single-base different sequences at 37 degrees C without the need for temperature adjustment. |
| 语种 | 英语 |
| WOS记录号 | WOS:000304919200011 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/49075]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhao, Meiping |
| 作者单位 | Peking Univ, Coll Chem & Mol Engn, MOE Key Lab Bioorgan Chem & Mol Engn, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiao, Xianjin,Zhang, Chen,Su, Xin,et al. A universal mismatch-directed signal amplification platform for ultra-selective and sensitive DNA detection under mild isothermal conditions[J]. CHEMICAL SCIENCE,2012,3(7):2257-2261. |
| APA | Xiao, Xianjin,Zhang, Chen,Su, Xin,Song, Chen,&Zhao, Meiping.(2012).A universal mismatch-directed signal amplification platform for ultra-selective and sensitive DNA detection under mild isothermal conditions.CHEMICAL SCIENCE,3(7),2257-2261. |
| MLA | Xiao, Xianjin,et al."A universal mismatch-directed signal amplification platform for ultra-selective and sensitive DNA detection under mild isothermal conditions".CHEMICAL SCIENCE 3.7(2012):2257-2261. |
入库方式： OAI收割
来源：化学研究所
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