Synthesis and Characterization of Oligofluorene Nanoparticles for Cell Imaging
文献类型：期刊论文
| 作者 | Wen Quanshan; Tang Hongwei; Yang Gaomai; Liu Libing; Lv Fengting; Yang Qiong; Wang Shu |
| 刊名 | ACTA CHIMICA SINICA
 |
| 出版日期 | 2012-10-28 |
| 卷号 | 70期号:20页码:2137-2143 |
| 关键词 | Conjugated Oligomer Nanoparticles Cellular Imaging Lysosome |
| ISSN号 | 0567-7351 |
| DOI | 10.6023/A12070461 |
| 英文摘要 | A new hydrophobic oligofluorene (OF) is synthesized via Suzuki cross-coupling reaction. The absorption and fluorescence spectra are measured in chloroforrn with high molar absorption coefficient (1.08 X 105 mol(-1.)L(.)cm(-1)) and fluorescence quantum yield (0.96). OF is dispersed into water to form nanoparticles with average size of 230 nm from dynamic light scattering (DLS) measurement. The nanoparticles in water still possess high molar absorption coefficient and fluorescence quantum yield. The quantitative cytotoxicity of OF nanoparticles to human lung cancer (A549) cells is measured by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method, showing that OF nanoparticles exhibit low cellular toxicity. OF nanoparticles can be utilized for imaging of live A549 cells. Confocal laser scanning microscopy demonstrates that OF nanoparticles are exclusively located in the cytoplasm, especially around the perinuclear region. OF nanoparticles exhibit good overlap staining with lysosome-specific dye (Lyso Tracker Red), which demonstrates that OF nanoparticles could be used for lysosome-specific imaging. |
| 语种 | 英语 |
| WOS记录号 | WOS:000311533600006 |
| 出版者 | SCIENCE PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/49123]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Wen Quanshan |
| 作者单位 | Chinese Acad Sci, Key Lab Organ Solids, Inst Chem, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wen Quanshan,Tang Hongwei,Yang Gaomai,et al. Synthesis and Characterization of Oligofluorene Nanoparticles for Cell Imaging[J]. ACTA CHIMICA SINICA,2012,70(20):2137-2143. |
| APA | Wen Quanshan.,Tang Hongwei.,Yang Gaomai.,Liu Libing.,Lv Fengting.,...&Wang Shu.(2012).Synthesis and Characterization of Oligofluorene Nanoparticles for Cell Imaging.ACTA CHIMICA SINICA,70(20),2137-2143. |
| MLA | Wen Quanshan,et al."Synthesis and Characterization of Oligofluorene Nanoparticles for Cell Imaging".ACTA CHIMICA SINICA 70.20(2012):2137-2143. |
入库方式： OAI收割
来源：化学研究所
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