Surface Plasmon Resonance Imaging System with Fixed and Compact Optical Path, 2x Zoom Mechanism and Wide Variable Incident Angle
文献类型：期刊论文
| 作者 | Zhang Yi-Ming1; Chen Jun1; Liao Tao1; Xu Ji-Ying1; Chen Yi1,2 |
| 刊名 | CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
 |
| 出版日期 | 2012-02-10 |
| 卷号 | 33期号:2页码:251-256 |
| 关键词 | Surface Plasmon Resonance Imaging Setup Fixed-optical Wide Variable Incident Angle Rightangle Trapezoid Prism Coupler |
| ISSN号 | 0251-0790 |
| 英文摘要 | A new surface plasmon resonance imaging(SPRi) system was designed and fabricated using a right angle trapezoid prism as SPR coupler, which allows the fixation of left optical elements in a compact space and addition of a 1-2x zooming adjuster. This design simplifies the angle scanning mechanism, enables a very fine adjustment of incident angle from 40 degrees to 80 degrees at 0.001 degrees angle resolution, and makes the observation possible of size-variable chips(1-4 cm(2)), providing a lateral spatial resolution up to 3600 Point/cm(2). its sensitivity was evaluated with sucrose solutions, giving the data up to 7x10(-6) RIU(Refractive index unit). A linear relationship was obtained between resonance angle and sample refractive index, with correlation coefficients of 0.99953. The new system has been further validated in the immuno-reaction of BSA with anti-BSA, which enables to produce both of kinetic reaction curves and differentiated dot images. These results indicate that the newly designed SPRi system is highly promising for high-through binding affinity applications. |
| 语种 | 英语 |
| WOS记录号 | WOS:000301023400007 |
| 出版者 | HIGHER EDUCATION PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/49153]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Chen Yi |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Analyt Chem Living Biosyst, Inst Chem, Beijing 100190, Peoples R China 2.Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang Yi-Ming,Chen Jun,Liao Tao,et al. Surface Plasmon Resonance Imaging System with Fixed and Compact Optical Path, 2x Zoom Mechanism and Wide Variable Incident Angle[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2012,33(2):251-256. |
| APA | Zhang Yi-Ming,Chen Jun,Liao Tao,Xu Ji-Ying,&Chen Yi.(2012).Surface Plasmon Resonance Imaging System with Fixed and Compact Optical Path, 2x Zoom Mechanism and Wide Variable Incident Angle.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,33(2),251-256. |
| MLA | Zhang Yi-Ming,et al."Surface Plasmon Resonance Imaging System with Fixed and Compact Optical Path, 2x Zoom Mechanism and Wide Variable Incident Angle".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 33.2(2012):251-256. |
入库方式： OAI收割
来源：化学研究所
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