Regulation of Glucose Oxidase Activity through Interaction with Fullerene Derivatives
文献类型：期刊论文
| 作者 | Gao Yunyan1,2; Wang Zhongli1,2; Ou Zhize1,2; Li Yi3; Wang Xuesong3; Yang Guoqiang4 |
| 刊名 | CHINESE JOURNAL OF CHEMISTRY
 |
| 出版日期 | 2012-02-01 |
| 卷号 | 30期号:2页码:418-426 |
| 关键词 | Fullerene C60 Glucose Oxidase Partial Mixed-type Inhibition Mechanism Redox Mediator Electron Transfer |
| ISSN号 | 1001-604X |
| DOI | 10.1002/cjoc.201180479 |
| 英文摘要 | The 2-(hydroxymethyl)pyridine modified C60 (PY-C60) and methoxydiglycol modified C60 (MDG-C60) are synthesized using Bingel-Hirsch reaction and characterized by nuclear magnetic resonance (NMR) and mass spectra. PY-C60 and MDG-C60 can bind to glucose oxidase (GOx) and quench the fluorescence of tryptophan (Trp) residue in GOx through static mechanism. The conformation of GOx is disturbed after formation of complex with these fullerene derivatives. Kinetic analysis indicates that PY-C60 and MDG-C60 may affect the catalytic activity of GOx with a partial mixed-type inhibition mechanism. In the plasma glucose concentration range (3.65.2 mmol center dot L-1), PY-C60 may significantly accelerate the catalytic velocity of GOx, however, MDG-C60 exerts almost no obvious change to the initial velocity of GOx, suggesting that elaborate design of molecular structure of fullerene derivative is very important for regulating the biological activity of fullerene-enzyme complex. |
| 语种 | 英语 |
| WOS记录号 | WOS:000300674000039 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/49209]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Gao Yunyan |
| 作者单位 | 1.NW Polytech Univ, Minist Educ, Key Lab Space Appl Phys & Chem, Xian 710072, Shaanxi, Peoples R China 2.NW Polytech Univ, Sch Sci, Dept Appl Chem, Xian 710072, Shaanxi, Peoples R China 3.Chinese Acad Sci, Tech Inst Phys & Chem, Beijing 100190, Peoples R China 4.Chinese Acad Sci, Inst Chem, CAS Key Lab Photochem, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao Yunyan,Wang Zhongli,Ou Zhize,et al. Regulation of Glucose Oxidase Activity through Interaction with Fullerene Derivatives[J]. CHINESE JOURNAL OF CHEMISTRY,2012,30(2):418-426. |
| APA | Gao Yunyan,Wang Zhongli,Ou Zhize,Li Yi,Wang Xuesong,&Yang Guoqiang.(2012).Regulation of Glucose Oxidase Activity through Interaction with Fullerene Derivatives.CHINESE JOURNAL OF CHEMISTRY,30(2),418-426. |
| MLA | Gao Yunyan,et al."Regulation of Glucose Oxidase Activity through Interaction with Fullerene Derivatives".CHINESE JOURNAL OF CHEMISTRY 30.2(2012):418-426. |
入库方式： OAI收割
来源：化学研究所
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