The homogeneous fluorescence anisotropic sensing of salivary lysozyme using the 6-carboxyfluorescein-labeled DNA aptamer
文献类型：期刊论文
| 作者 | Zou, Mingjian; Chen, Yang; Xu, Xiao; Huang, Hongduan; Liu, Feng; Li, Na |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2012-02-15 |
| 卷号 | 32期号:1页码:148-154 |
| 关键词 | Fluorescence Anisotropy 6-carboxyfluorescein (Fam) Lysozyme Dna Aptamer Dynamic Fluorescence Anisotropy Lifetime |
| ISSN号 | 0956-5663 |
| DOI | 10.1016/j.bios.2011.11.052 |
| 英文摘要 | A simple and sensitive fluorescence anisotropy method was developed for lysozyme, employing the coupling of fluorophore, 6-carboxyfluorescein (FAM), with lysozyme upon recognition between the target molecule and its DNA aptamer. It was found in this study that the rotational dynamic of the detecting system is crucial to obtain a high anisotropy signal that cannot always be achieved by simply increasing the molecular volume, because molecular volume increase may not be able to efficiently retard the rotational movement of the fluorophore. FAM was selected as the label of the ssDNA aptamer to effectively facilitate the change of the fluorophore from a primarily independent segmental movement to slow global rotation. The time-resolved measurements, including lifetime and dynamic fluorescence anisotropy, were conducted to study the recognition interaction and to better understand the methodology. The proposed method had a wide linear dynamic range of 12.5-300 nM and a high sensitivity with the limit of detection of 4.9 nM (3S/N). This proposed method was successfully applied to assay of human salivary lysozyme. The results based on the standard addition recovery and comparison with enzyme-linked immunosorbent assay (ELISA) demonstrated the feasibility of this method for biological samples. Using coupling between the fluorophore and the analyte can be one of the approaches working toward expanding the application of fluorescence anisotropy based on aptamer-target and antibody-antigen recognitions. (C) 2011 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000301011100021 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/49249]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Li, Na |
| 作者单位 | Peking Univ, Beijing Natl Lab Mol Sci BNLMS, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Inst Analyt Chem,Coll Chem & Mol Engn, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zou, Mingjian,Chen, Yang,Xu, Xiao,et al. The homogeneous fluorescence anisotropic sensing of salivary lysozyme using the 6-carboxyfluorescein-labeled DNA aptamer[J]. BIOSENSORS & BIOELECTRONICS,2012,32(1):148-154. |
| APA | Zou, Mingjian,Chen, Yang,Xu, Xiao,Huang, Hongduan,Liu, Feng,&Li, Na.(2012).The homogeneous fluorescence anisotropic sensing of salivary lysozyme using the 6-carboxyfluorescein-labeled DNA aptamer.BIOSENSORS & BIOELECTRONICS,32(1),148-154. |
| MLA | Zou, Mingjian,et al."The homogeneous fluorescence anisotropic sensing of salivary lysozyme using the 6-carboxyfluorescein-labeled DNA aptamer".BIOSENSORS & BIOELECTRONICS 32.1(2012):148-154. |
入库方式： OAI收割
来源：化学研究所
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