An electrochemical aptasensor based on enzyme linked aptamer assay
文献类型：期刊论文
| 作者 | Zhang, De-Wen1; Sun, Cui-Jin2; Zhang, Fang-Ting1; Xu, Li3; Zhou, Ying-Lin1; Zhang, Xin-Xiang1 |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2012-01-15 |
| 卷号 | 31期号:1页码:363-368 |
| 关键词 | Enzyme Linked Aptamer Assay Aptamer Fragment Cocaine Streptavidin-hrp Differential Pulse Voltammetry |
| ISSN号 | 0956-5663 |
| DOI | 10.1016/j.bios.2011.10.046 |
| 英文摘要 | An aptamer is an artificial functional oligonucleic acid, which can interact with its target molecule with high affinity and specificity. Enzyme linked aptamer assay (ELAA) is developed to detect cocaine using aptamer fragment/cocaine configuration based on the affinity interaction between aptamer fragments with cocaine. The aptasensor was constructed by cleaving anticocaine aptamer into two fragments: one was assembled on a gold electrode surface, while the other was modified with biotin at 3'-end, which could be further labelled with streptavidin-horseradish peroxidase (SA-HRP). Upon binding with cocaine, the HRP-labelled aptamer fragment/cocaine complex formed on the electrode would increase the reduction current of hydroquinone (HQ) in the presence of H2O2. The sensitivity and the specificity of the proposed electrochemical aptasensor were investigated by differential pulse voltammetry (DPV). The results indicated that the DPV signal change could be used to sensitively detect cocaine with the dynamic range from 0.1 mu M to 50 mu M and the detection limit down to 20 nM (S/N = 3). The proposed aptasensor has the advantages of high sensitivity and low background current. Furthermore, a new configuration for ELAA requiring only a single aptamer sequence is constructed, which can be generalized for detecting different kinds of targets by cleaving the aptamers into two suitable segments. (C) 2011 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000300468400056 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/49451]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhou, Ying-Lin |
| 作者单位 | 1.Peking Univ, BNLMS, Key Lab Biochem & Mol Engn, Coll Chem, Beijing 100871, Peoples R China 2.Univ Int Relat, Dept Appl Chem, Beijing 100091, Peoples R China 3.Beijing Inst Microchem, Beijing 100091, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, De-Wen,Sun, Cui-Jin,Zhang, Fang-Ting,et al. An electrochemical aptasensor based on enzyme linked aptamer assay[J]. BIOSENSORS & BIOELECTRONICS,2012,31(1):363-368. |
| APA | Zhang, De-Wen,Sun, Cui-Jin,Zhang, Fang-Ting,Xu, Li,Zhou, Ying-Lin,&Zhang, Xin-Xiang.(2012).An electrochemical aptasensor based on enzyme linked aptamer assay.BIOSENSORS & BIOELECTRONICS,31(1),363-368. |
| MLA | Zhang, De-Wen,et al."An electrochemical aptasensor based on enzyme linked aptamer assay".BIOSENSORS & BIOELECTRONICS 31.1(2012):363-368. |
入库方式： OAI收割
来源：化学研究所
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