PEGylation of bovine serum albumin using click chemistry for the application as drug carriers
文献类型：期刊论文
| 作者 | Li, Xiao-Yuan1; Li, Tai-Hang1,2; Guo, Jin-Shan1; Wei, Ying1,2; Jing, Xia-Bin1; Chen, Xue-Si1; Huang, Yu-Bin1 |
| 刊名 | BIOTECHNOLOGY PROGRESS
 |
| 出版日期 | 2012-05-01 |
| 卷号 | 28期号:3页码:856-861 |
| 关键词 | Bovine Serum Albumin Mpeg Click Chemistry Rifampicin 5-fluorouracil Drug Carriers |
| ISSN号 | 8756-7938 |
| DOI | 10.1002/btpr.1526 |
| 英文摘要 | Monomethyl poly(ethylene glycol) (mPEG)-modified bovine serum albumin (BSA) conjugates (BSA-mPEG) were obtained by the mild Cu(I)-mediated cycloaddition reaction of azided BSA (BSA-N3) and alkyne-terminated mPEG. The structure and characteristics of BSA-mPEG conjugates were thoroughly investigated. There were about two PEG chains conjugated onto each BSA molecule as determined by matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) analysis. The intrinsic nonspecific binding ability of BSA was used for adsorption and sustained release of both rifampicn and 5-fluorouracil (5-FU). The helical structures of BSA were preserved to a large extent after modification and drug adsorption on BSA was confirmed via circular dichroism spectroscopy. Drugs adsorbed onto the conjugated formulation to a lesser extent than on BSA due to mPEG modification. The in vitro release of both rifampicin and 5-FU, however, indicated that BSA-mPEG can function as a drug carrier. Overall, the click reaction provided a convenient tool for the pegylation of BSA. The biological activity of the BSA-mPEG conjugates, including the drug transportation capacity and biocompatibility, were largely retained. (C) 2012 American Institute of Chemical Engineers Biotechnol. Prog.,, 2012 |
| 语种 | 英语 |
| WOS记录号 | WOS:000304989800026 |
| 出版者 | WILEY-BLACKWELL |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/49565]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Huang, Yu-Bin |
| 作者单位 | 1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Changchun Inst Appl Chem, Chinese Acad Sci, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Xiao-Yuan,Li, Tai-Hang,Guo, Jin-Shan,et al. PEGylation of bovine serum albumin using click chemistry for the application as drug carriers[J]. BIOTECHNOLOGY PROGRESS,2012,28(3):856-861. |
| APA | Li, Xiao-Yuan.,Li, Tai-Hang.,Guo, Jin-Shan.,Wei, Ying.,Jing, Xia-Bin.,...&Huang, Yu-Bin.(2012).PEGylation of bovine serum albumin using click chemistry for the application as drug carriers.BIOTECHNOLOGY PROGRESS,28(3),856-861. |
| MLA | Li, Xiao-Yuan,et al."PEGylation of bovine serum albumin using click chemistry for the application as drug carriers".BIOTECHNOLOGY PROGRESS 28.3(2012):856-861. |
入库方式： OAI收割
来源：化学研究所
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