中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Detection of CD4(+) T-lymphocytes from hemodialyzed patients by surface plasmon resonance

文献类型:期刊论文

作者Wang, Hai Yan1; Li, Jian Jun2; Cao, Xiao Na3; Xu, Ji Ying4; Liu, Mei Rong4; Chen, Yi4
刊名CHINESE CHEMICAL LETTERS
出版日期2012-12-01
卷号23期号:12页码:1393-1395
关键词Hemodialyzed Patients Cd4(+) T-lymphocytes Antibody-antigen Interactions Spr
ISSN号1001-8417
DOI10.1016/j.cclet.2012.10.017
英文摘要A surface plasmon resonance (SPR) method was presented to discriminate hemodialyzed T-lymphocytes from the normal based on antibody cell recognition. By dynamic reaction with fixed anti-human CD4 antibody, SPR could offer significant signals to distinguish hemodialyzed patients from the healthy Controls within 200 s after the cell injection in respect of either rising speed or maximum binding capacity (p < 0.01). The ratio method is also used to exclude the non-specific adsorption. The percentage of hemodialyzed patients' CD4(+) T cells against the healthy control is 69 +/- 18%. The most attractive of the present method is its ability to detect the intact and label-free lymphocytes, and further to detect the subpopulations, or proteins secreted by the desired lymphocytes subset. (C) 2012 Yi Chen. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.
语种英语
WOS记录号WOS:000313999900021
出版者ELSEVIER SCIENCE INC
源URL[http://ir.iccas.ac.cn/handle/121111/49577]  
专题中国科学院化学研究所
通讯作者Chen, Yi
作者单位1.Shanxi Datong Univ, Sch Chem & Chem Engn, Datong 037009, Peoples R China
2.Dongzhimen Hosp Affiliated, East Sect, Beijing 101100, Peoples R China
3.Being Sport Univ, Community Hlth Serv, Beijing 100190, Peoples R China
4.ICCAS, Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China
推荐引用方式
GB/T 7714
Wang, Hai Yan,Li, Jian Jun,Cao, Xiao Na,et al. Detection of CD4(+) T-lymphocytes from hemodialyzed patients by surface plasmon resonance[J]. CHINESE CHEMICAL LETTERS,2012,23(12):1393-1395.
APA Wang, Hai Yan,Li, Jian Jun,Cao, Xiao Na,Xu, Ji Ying,Liu, Mei Rong,&Chen, Yi.(2012).Detection of CD4(+) T-lymphocytes from hemodialyzed patients by surface plasmon resonance.CHINESE CHEMICAL LETTERS,23(12),1393-1395.
MLA Wang, Hai Yan,et al."Detection of CD4(+) T-lymphocytes from hemodialyzed patients by surface plasmon resonance".CHINESE CHEMICAL LETTERS 23.12(2012):1393-1395.

入库方式: OAI收割

来源:化学研究所

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