Detection of CD4(+) T-lymphocytes from hemodialyzed patients by surface plasmon resonance
文献类型：期刊论文
| 作者 | Wang, Hai Yan1; Li, Jian Jun2; Cao, Xiao Na3; Xu, Ji Ying4; Liu, Mei Rong4; Chen, Yi4 |
| 刊名 | CHINESE CHEMICAL LETTERS
 |
| 出版日期 | 2012-12-01 |
| 卷号 | 23期号:12页码:1393-1395 |
| 关键词 | Hemodialyzed Patients Cd4(+) T-lymphocytes Antibody-antigen Interactions Spr |
| ISSN号 | 1001-8417 |
| DOI | 10.1016/j.cclet.2012.10.017 |
| 英文摘要 | A surface plasmon resonance (SPR) method was presented to discriminate hemodialyzed T-lymphocytes from the normal based on antibody cell recognition. By dynamic reaction with fixed anti-human CD4 antibody, SPR could offer significant signals to distinguish hemodialyzed patients from the healthy Controls within 200 s after the cell injection in respect of either rising speed or maximum binding capacity (p < 0.01). The ratio method is also used to exclude the non-specific adsorption. The percentage of hemodialyzed patients' CD4(+) T cells against the healthy control is 69 +/- 18%. The most attractive of the present method is its ability to detect the intact and label-free lymphocytes, and further to detect the subpopulations, or proteins secreted by the desired lymphocytes subset. (C) 2012 Yi Chen. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000313999900021 |
| 出版者 | ELSEVIER SCIENCE INC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/49577]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Chen, Yi |
| 作者单位 | 1.Shanxi Datong Univ, Sch Chem & Chem Engn, Datong 037009, Peoples R China 2.Dongzhimen Hosp Affiliated, East Sect, Beijing 101100, Peoples R China 3.Being Sport Univ, Community Hlth Serv, Beijing 100190, Peoples R China 4.ICCAS, Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Hai Yan,Li, Jian Jun,Cao, Xiao Na,et al. Detection of CD4(+) T-lymphocytes from hemodialyzed patients by surface plasmon resonance[J]. CHINESE CHEMICAL LETTERS,2012,23(12):1393-1395. |
| APA | Wang, Hai Yan,Li, Jian Jun,Cao, Xiao Na,Xu, Ji Ying,Liu, Mei Rong,&Chen, Yi.(2012).Detection of CD4(+) T-lymphocytes from hemodialyzed patients by surface plasmon resonance.CHINESE CHEMICAL LETTERS,23(12),1393-1395. |
| MLA | Wang, Hai Yan,et al."Detection of CD4(+) T-lymphocytes from hemodialyzed patients by surface plasmon resonance".CHINESE CHEMICAL LETTERS 23.12(2012):1393-1395. |
入库方式： OAI收割
来源：化学研究所
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