Rational design of true monomeric and bright photoactivatable fluorescent proteins
文献类型：期刊论文
| 作者 | Zhang, Mingshu1,2; Chang, Hao1,3; Zhang, Yongdeng1,4; Yu, Junwei1,4; Wu, Lijie1; Ji, Wei1; Chen, Juanjuan5; Liu, Bei1,4; Lu, Jingze1; Liu, Yingfang1 |
| 刊名 | NATURE METHODS
 |
| 出版日期 | 2012-07-01 |
| 卷号 | 9期号:7页码:727-U297 |
| ISSN号 | 1548-7091 |
| DOI | 10.1038/NMETH.2021 |
| 英文摘要 | Monomeric (m) Eos2 is an engineered photoactivatable fluorescent protein widely used for super-resolution microscopy. We show that mEos2 forms oligomers at high concentrations and forms aggregates when labeling membrane proteins, limiting its application as a fusion partner. We solved the crystal structure of tetrameric mEos2 and rationally designed improved versions, mEos3.1 and mEos3.2, that are truly monomeric, are brighter, mature faster and exhibit higher photon budget and label density. |
| 语种 | 英语 |
| 出版者 | NATURE PUBLISHING GROUP |
| WOS记录号 | WOS:000305942200030 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/49693]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Xu, Pingyong |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China 4.Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Wuhan 430074, Hubei, Peoples R China 5.Peking Univ, Coll Chem & Mol Engn, State Key Lab Rare Earth Mat Chem & Applicat, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Mingshu,Chang, Hao,Zhang, Yongdeng,et al. Rational design of true monomeric and bright photoactivatable fluorescent proteins[J]. NATURE METHODS,2012,9(7):727-U297. |
| APA | Zhang, Mingshu.,Chang, Hao.,Zhang, Yongdeng.,Yu, Junwei.,Wu, Lijie.,...&Xu, Tao.(2012).Rational design of true monomeric and bright photoactivatable fluorescent proteins.NATURE METHODS,9(7),727-U297. |
| MLA | Zhang, Mingshu,et al."Rational design of true monomeric and bright photoactivatable fluorescent proteins".NATURE METHODS 9.7(2012):727-U297. |
入库方式： OAI收割
来源：化学研究所
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